Life Science Research Center, Gifu University, Gifu 501-1193, Japan.
Fungal Biol. 2011 Dec;115(12):1244-50. doi: 10.1016/j.funbio.2011.08.009. Epub 2011 Sep 13.
Fusarium asiaticum strains resistant to thiophanate-methyl were detected in four prefectures in Japan though their proportion in the total population was low in all instances. The F167Y or F200Y mutation in the β2-tubulin gene (FGSG06611.3) was detected in thiophanate-methyl resistant (TMR) strains. A PCR-based diagnostic method based on these mutations was developed and applied for all 17 TMR strains that have been detected so far in Japan. Three and 14 TMR strains were the F167Y and F200Y mutation types, respectively. Analysis by 11 variable number of tandem repeat markers showed that TMR strains from the same site had an identical genotype, while TMR strains from different sites were dissimilar. This result indicates that TMR strains did not spread clonally to the different sites.
在日本的四个县检测到了对硫菌灵具有抗性的亚洲镰刀菌菌株，尽管它们在所有情况下在总种群中的比例都很低。在β2-微管蛋白基因（FGSG06611.3）中检测到 F167Y 或 F200Y 突变的菌株对硫菌灵具有抗性（TMR）。基于这些突变开发了一种基于 PCR 的诊断方法，并应用于迄今为止在日本检测到的所有 17 株 TMR 菌株。3 株和 14 株 TMR 菌株分别为 F167Y 和 F200Y 突变类型。通过 11 个可变串联重复标记的分析表明，来自同一地点的 TMR 菌株具有相同的基因型，而来自不同地点的 TMR 菌株则不同。这一结果表明，TMR 菌株并没有以无性方式传播到不同的地点。
