United Graduate School of Agricultural Science, Gifu University, Gifu 501-1193, Japan.
Graduate School of Bioresources, Mie University, Tsu, Mie 514-8507, Japan.
Plant Dis. 2022 Feb;106(2):634-640. doi: 10.1094/PDIS-07-21-1501-RE. Epub 2022 Feb 8.
is the pathogen of rice bakanae disease and is subclassified into gibberellin and fumonisin groups (G and F groups). Thiophanate-methyl (TM), a benzimidazole fungicide, has been used extensively to control Previous investigation showed that F-group strains are TM sensitive (TMS), whereas most G-group strains are TM resistant (TMR) in Japan. The minimum inhibitory concentration in TMS strains was 1 to 10 μg ml, whereas that in TMR strains was >100 μg ml. E198K and F200Y mutations in β-tubulin were detected in TMR strains. A loop-mediated isothermal amplification-fluorescent loop primer method was developed for diagnosis of these mutations and applied to 37 TMR strains and 56 TMS strains. The results indicated that 100% of TMR strains were identified as having either the E198K mutation (41%) or the F200Y mutation (59%), whereas none of the TMS strains tested showed either mutation. We found one remarkable TMR strain in the F group that had an F200Y mutation. These results suggest that E198K and F200Y mutations in β-tubulin contribute to TM resistance in
是水稻恶苗病的病原体，分为赤霉素和伏马菌素两个亚组（G 和 F 组）。多菌灵（TM）是一种苯并咪唑类杀菌剂，广泛用于防治日本的水稻恶苗病。先前的调查表明，F 组菌株对多菌灵敏感（TMS），而大多数 G 组菌株对多菌灵具有抗性（TMR）。TMS 菌株的最低抑菌浓度为 1 至 10μg/ml，而 TMR 菌株的最低抑菌浓度则>100μg/ml。在 TMR 菌株中检测到β-微管蛋白中的 E198K 和 F200Y 突变。开发了一种环介导等温扩增-荧光环引物方法用于这些突变的诊断，并应用于 37 株 TMR 菌株和 56 株 TMS 菌株。结果表明，100%的 TMR 菌株被鉴定为要么具有 E198K 突变（41%），要么具有 F200Y 突变（59%），而没有一株 TMS 菌株显示出这两种突变。我们在 F 组中发现了一株具有 F200Y 突变的显著 TMR 菌株。这些结果表明，β-微管蛋白中的 E198K 和 F200Y 突变导致对多菌灵的抗性。
