Cancer Center, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, China.
Cancer Immunol Immunother. 2012 Jun;61(6):911-6. doi: 10.1007/s00262-011-1158-4. Epub 2011 Nov 25.
Expression levels of VEGF and Her-2, levels of T-regulatory (Treg) cells, levels of CD3+ cells, and ratios of Th (CD4+ T cells)/Tr (Treg) cells were compared between stage I, II, III, and IV breast cancer patients (n = 120) prior to chemotherapy and healthy women (n = 30). Cells from peripheral blood were counted by flow cytometry, Her-2 and VEGF expression was detected by pathological examination, and Her-2 was detected by FISH. Breast cancer patients had more Treg cells and a lower ratio of Th/Tr cells than the healthy women. Stage IV breast cancer patients had more Treg cells and a lower ratio of Th/Tr cells than stage I, II, or III breast cancer patients. Patients positive for VEGF had a lower ratio of Th/Tr cells compared with patients negative for VEGF, and those positive for both VEGF and Her-2 also had a lower ratio of Th/Tr cells compared with patients not positive for both VEGF and Her-2. The decreased Th/Tr cells ratio indicates impaired immune function, suggesting that the stage IV breast cancer and the Her-2/VEGF-positive breast cancer patients have lower immune function.
比较了化疗前 I 期、II 期、III 期和 IV 期乳腺癌患者（n=120）和健康女性（n=30）的 VEGF 和 Her-2 表达水平、T 调节（Treg）细胞水平、CD3+细胞水平以及 Th（CD4+T 细胞）/Tr（Treg）细胞比值。通过流式细胞术计数外周血中的细胞，通过病理检查检测 Her-2 和 VEGF 的表达，并通过 FISH 检测 Her-2。与健康女性相比，乳腺癌患者的 Treg 细胞更多，Th/Tr 细胞比值更低。与 I 期、II 期或 III 期乳腺癌患者相比，IV 期乳腺癌患者的 Treg 细胞更多，Th/Tr 细胞比值更低。与 VEGF 阴性患者相比，VEGF 阳性患者的 Th/Tr 细胞比值更低，与既不是 VEGF 阳性也不是 Her-2 阳性的患者相比，同时也是 VEGF 和 Her-2 阳性的患者的 Th/Tr 细胞比值也更低。Th/Tr 细胞比值的降低表明免疫功能受损，这表明 IV 期乳腺癌和 Her-2/VEGF 阳性乳腺癌患者的免疫功能较低。
