Ramelot Theresa A, Yang Yunhuang, Xiao Rong, Acton Thomas B, Everett John K, Montelione Gaetano T, Kennedy Michael A
Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056 and the Northeast Structural Genomics Consortium.
Proteins. 2012 Feb;80(2):667-70. doi: 10.1002/prot.23235. Epub 2011 Nov 24.
Here we describe the solution NMR structure of the 120 amino acid fragment of BT_0084, without the N-terminal lipoprotein targeting sequence, encoded in a conjugative transposon (CTn) in the genome of Bacteroides thetaiotamicron. BT_0084 belongs to a conserved family of TraQ lipoproteins that are encoded at the end of the tra operon, which contains genes essential for transfer of CTns. The structure belongs to the immunoglobulin superfamily and shares structural similarity, albeit low sequence identity (< 15%), to other proteins involved in pili production for bacterial cell attachment. Although its role in repression of CTn transfer remains to be determined, the structure of BT_0084 reported here represents the first from the Bacteroides TraQ family and should facilitate further understanding of the tra operon-regulated transfer of CTns.
在此，我们描述了来自多形拟杆菌基因组中一个接合转座子（CTn）编码的BT_0084的120个氨基酸片段的溶液核磁共振结构，该片段不含N端脂蛋白靶向序列。BT_0084属于TraQ脂蛋白的一个保守家族，该家族编码于tra操纵子末端，tra操纵子包含CTn转移所必需的基因。该结构属于免疫球蛋白超家族，与其他参与细菌细胞附着菌毛产生的蛋白质在结构上具有相似性，尽管序列同一性较低（<15%）。尽管其在抑制CTn转移中的作用仍有待确定，但此处报道的BT_0084的结构代表了拟杆菌TraQ家族中的首个结构，应有助于进一步了解tra操纵子调控的CTn转移。
