Department of Microbiology, Nantong Center for Disease Control and Prevention, Nantong, Jiangsu, China.
Epidemiol Infect. 2012 Sep;140(9):1607-11. doi: 10.1017/S0950268811002433. Epub 2011 Nov 25.
Measles control in China is monitored in part by surveillance of circulating wild-type viruses. The objective of this study was genetic characterization and phylogenetic analysis of measles strains in the Nantong City region of Jiangsu province, China, during 2010. Sera from suspected cases were tested for IgM antibodies and measles virus isolated by inoculation of transport medium onto Vero/SLAM cells. Isolated strains were phylogenetically analysed according to the nucleotide sequence of the C-terminal region of the nucleoprotein gene amplified by RT-PCR. The results revealed 34 cases confirmed by positive IgM, for an incidence of 0·45/100 000. Six isolates identified were all clustered within genotype H1. The findings reported here support continued endemic transmission of measles virus in China.
中国的麻疹控制部分通过监测循环野病毒来监测。本研究的目的是对 2010 年江苏省南通市麻疹病毒株进行遗传特征分析和系统进化分析。采集疑似病例的血清，通过接种运输培养基到 Vero/SLAM 细胞来检测 IgM 抗体和麻疹病毒。根据通过 RT-PCR 扩增的核蛋白基因 C 末端区的核苷酸序列对分离株进行系统进化分析。结果显示，34 例经 IgM 阳性确诊，发病率为 0.45/100000。鉴定的 6 个分离株均属于基因型 H1。本研究结果支持中国麻疹病毒持续地方性传播。
