Zhang F, Chen L, Qin J, Zhao W, Wu P
College of Life Science, East China Normal University, Shanghai 200062, China.
Genetika. 2011 Sep;47(9):1190-5.
A novel gene encoding avon Willebrand factor D (vWD) domain and three Kazal-type domains was firstly indentified from the ovary of the oriental river prawn Macrobrachium nipponense and this gene was named as MnvWD-Kazal. Bioinformatics analyses showed that this gene encodes a protein of 857 amino acids with a predicted molecular mass of 92.7 kDa. Real-time quantitative PCR (RT-QPCR) analyses revealed that the level of MnvWD-Kazal mRNA expression varied in the developing ovary and substantially differed between other tissues. In the ovary, the level of MnvWD-Kazal expression gradually increased from the perinucleolus (PN) stage to the yolk granule (YG) stage, and then abruptly decreased at the sexual maturation (MA) stage. The maximum expression occurred in the YG stage and the minimum was at the paracmasis (PM) stage. The expression level of MnvWD-Kazal in the intestine was much higher than that in other tissues. The differential expressions of MnvWD-Kazal at different stages of the ovary suggest that this novel gene may play a critical role in the oocyte maturation of M. nipponense.
首次从日本沼虾卵巢中鉴定出一个编码血管性血友病因子D（vWD）结构域和三个Kazal型结构域的新基因，该基因被命名为MnvWD-Kazal。生物信息学分析表明，该基因编码一个由857个氨基酸组成的蛋白质，预测分子量为92.7 kDa。实时定量PCR（RT-QPCR）分析显示，MnvWD-Kazal mRNA在发育中的卵巢中的表达水平有所不同，且在其他组织之间存在显著差异。在卵巢中，MnvWD-Kazal的表达水平从核仁周围（PN）期到卵黄颗粒（YG）期逐渐升高，然后在性成熟（MA）期突然下降。最高表达出现在YG期，最低表达出现在产卵后期（PM）。MnvWD-Kazal在肠道中的表达水平远高于其他组织。MnvWD-Kazal在卵巢不同阶段的差异表达表明，这个新基因可能在日本沼虾的卵母细胞成熟过程中起关键作用。
