College of Life Science, East China Normal University, Shanghai 200062, China; East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.
Comp Biochem Physiol B Biochem Mol Biol. 2010 Mar;155(3):288-93. doi: 10.1016/j.cbpb.2009.11.013. Epub 2009 Nov 26.
The small ubiquitin-like modifier (SUMO) pathway in eukaryotes is an essential biological process involving cellular processes, development and organelle biogenesis. In a sequential enzymatic action, Ubc9 is an important conjunction enzyme in the SUMO pathway. Although the Ubc9 has been found in vertebrates, its expression in crustaceans is little known. In this study, the Ubc9 was identified in the embryo and ovary of a freshwater prawn Macrobrachium nipponense for the first time and it was denoted as MnUbc9. Bioinformatics analyses showed that this gene encodes a protein of 161 amino acids with predicted molecular mass of 18.32kDa. Real-time quantitative PCR analyses demonstrated that the expression levels varied significantly in the developing embryo and ovary. In the embryo, the expression level of MnUbc9 was higher at the cleavage stage (CS) than at the blastula stage (BS), and reached even higher levels at the protozoea stage (PS) and the zoea stage (ZS). In the ovary, the MuUbc9 expression was low at the early stage, but reached the highest at the yolk granule stage (YG), and then abruptly declined at the maturation stage (MA). The differential expressions of MnUbc9 in the embryo and ovary suggest that MnUbc9 may play an important role in embryogenesis and oogenesis of M. nipponense.
真核生物中的小泛素样修饰物（SUMO）途径是一个涉及细胞过程、发育和细胞器发生的重要生物学过程。在连续的酶促反应中，Ubc9 是 SUMO 途径中的一个重要连接酶。尽管在脊椎动物中已经发现了 Ubc9，但在甲壳类动物中的表达却鲜为人知。在本研究中，首次在淡水虾 Macrobrachium nipponense 的胚胎和卵巢中鉴定到 Ubc9，并将其命名为 MnUbc9。生物信息学分析表明，该基因编码一个由 161 个氨基酸组成的蛋白质，预测分子量为 18.32kDa。实时定量 PCR 分析表明，该基因在发育中的胚胎和卵巢中的表达水平存在显著差异。在胚胎中，MnUbc9 的表达水平在卵裂期（CS）高于囊胚期（BS），在原肠胚期（PS）和无节幼体期（ZS）达到更高水平。在卵巢中，MuUbc9 的表达水平在早期较低，但在卵黄颗粒期（YG）达到最高水平，然后在成熟期（MA）急剧下降。MnUbc9 在胚胎和卵巢中的差异表达表明，MnUbc9 可能在 M. nipponense 的胚胎发生和卵子发生中发挥重要作用。
