Gao L, Cheng H Y, Dong L, Ye X, Liu Y N, Chang X H, Cheng Y X, Chen J, Ma R Q, Cui H
Gynecological Oncology Centre, Peking University People's Hospital, Beijing, China.
J Int Med Res. 2011;39(5):1645-60. doi: 10.1177/147323001103900507.
As a promising biomarker, human epididymis protein 4 (HE4) has been widely used for the early detection and differential diagnosis of ovarian cancer. This study evaluated the function of HE4 in the carcinogenesis and progression of ovarian cancer. An enzyme immunometric assay, used to detect HE4 in the serum of ovarian cancer patients, showed that the protein could discriminate between malignant and benign ovarian tumours with high specificity. An exogenous HE4 gene was transfected into ovarian cancer cell lines and an immortalized ovarian epithelial cell line. Compared with the controls, HE4 overexpression significantly promoted cell apoptosis and adhesion. Overexpression of HE4 also led to significant inhibition of cell proliferation, migration and invasiveness in vitro, as well as xenograft tumour formation in vivo. This is the first report to demonstrate the functional importance of HE4 in multiple cellular processes and indicates that HE4 may play a protective role in the progression of ovarian cancer.
作为一种很有前景的生物标志物，人附睾蛋白4（HE4）已被广泛用于卵巢癌的早期检测和鉴别诊断。本研究评估了HE4在卵巢癌发生发展过程中的作用。一种用于检测卵巢癌患者血清中HE4的酶免疫测定法显示，该蛋白能够高度特异性地区分恶性和良性卵巢肿瘤。将外源性HE4基因转染到卵巢癌细胞系和永生化卵巢上皮细胞系中。与对照组相比，HE4过表达显著促进细胞凋亡和黏附。HE4过表达还导致体外细胞增殖、迁移和侵袭以及体内异种移植肿瘤形成受到显著抑制。这是首次证明HE4在多个细胞过程中功能重要性的报告，表明HE4可能在卵巢癌进展中起保护作用。
