Saito E, Mukai M, Muraki T, Ichikawa Y, Homma M
Endocrinology. 1979 Feb;104(2):487-92. doi: 10.1210/endo-104-2-487.
Monolayer cultures of mouse adrenal tumor cell line Y-1 have been used to investigate the effects of glucocorticoid on cell replication, [3H]thymidine incorporation into the trichloroacetic acid-precipitated cell fraction, steroidogenesis, and the ACTH receptors of adrenocortical cells. Corticosterone at a concentration of 5.0--50 micrograms/ml inhibited cell replication and [3H]thymidine incorporation into trichloroacetic acid-precipitated cell fraction in a dose-related manner. Corticosterone at a concentration of 0.5--50 micrograms/ml inhibited ACTH-induced steroidogenesis in a dose-related manner. Steroids which do not possess glucocorticoid action did not show such inhibitory effects on cell replication and steroidogenesis of Y-1 cells. The characteristics of the ACTH receptors of these cells remained unaffected by corticosterone. Our findings suggest that synthesized or secreted glucocorticoid may play an important role in the direct regulation of proliferation and function of adrenocortical cells under physiological conditions.
小鼠肾上腺肿瘤细胞系Y-1的单层培养物已被用于研究糖皮质激素对细胞复制、[3H]胸腺嘧啶核苷掺入三氯乙酸沉淀的细胞组分、类固醇生成以及肾上腺皮质细胞促肾上腺皮质激素（ACTH）受体的影响。浓度为5.0--50微克/毫升的皮质酮以剂量相关的方式抑制细胞复制和[3H]胸腺嘧啶核苷掺入三氯乙酸沉淀的细胞组分。浓度为0.5--50微克/毫升的皮质酮以剂量相关的方式抑制ACTH诱导的类固醇生成。不具有糖皮质激素作用的类固醇对Y-1细胞的细胞复制和类固醇生成未显示出此类抑制作用。这些细胞的ACTH受体特性不受皮质酮影响。我们的研究结果表明，合成或分泌的糖皮质激素在生理条件下可能在肾上腺皮质细胞增殖和功能的直接调节中发挥重要作用。
