促肾上腺皮质激素通过3':5'-环磷酸腺苷介导对功能性小鼠肾上腺肿瘤细胞复制的抑制作用。
Inhibition of replication in functional mouse adrenal tumor cells by adrenocorticotropic hormone mediated by adenosine 3':5'-cyclic monophosphate.
作者信息
Masui H, Garren L D
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3206-10. doi: 10.1073/pnas.68.12.3206.
Adrenocorticotropic hormone (ACTH) inhibited replication in functional adrenal tumor cells with a concomitant stimulation of steroidogenesis and a characteristic change of morphology from a flattened to a spherical type. [(3)H]Thymidine incorporation into DNA was inhibited by about 50% 6 hours after ACTH treatment. Both cyclic AMP and dibutyryl cyclic AMP inhibited [(3)H]thymidine incorporation and caused the characteristic morphological change noted with ACTH. The extent of stimulation of steroidogenesis and the amount of inhibition of [(3)H]thymidine incorporation in response to various doses of ACTH were closely related and both were in parallel with the concentration of cyclic AMP in the cells. Cyclic GMP and cyclic IMP did not inhibit [(3)H]thymidine incorporation significantly, and did not change the morphology of the cells. AMP inhibited [(3)H]thymidine incorporation into DNA and caused the characteristic morphological change. However, AMP did not increase the cyclic AMP content of the cells. CMP, GMP, and UMP showed a significant inhibition of [(3)H]thymidine incorporation into DNA, but the extent of the inhibition was much less than that with AMP. These nucleotides did not change the morphology of the cells.
促肾上腺皮质激素（ACTH）抑制功能性肾上腺肿瘤细胞的复制，同时刺激类固醇生成，并使细胞形态从扁平型向球形发生特征性改变。促肾上腺皮质激素处理6小时后，[³H]胸腺嘧啶核苷掺入DNA的量被抑制了约50%。环磷酸腺苷（cAMP）和二丁酰环磷酸腺苷（dbcAMP）均抑制[³H]胸腺嘧啶核苷掺入，并引起促肾上腺皮质激素所导致的特征性形态变化。不同剂量的促肾上腺皮质激素刺激类固醇生成的程度以及对[³H]胸腺嘧啶核苷掺入的抑制量密切相关，且二者均与细胞内环磷酸腺苷的浓度呈平行关系。环磷酸鸟苷（cGMP）和环磷酸肌苷（cIMP）对[³H]胸腺嘧啶核苷掺入无显著抑制作用，也不改变细胞形态。腺苷一磷酸（AMP）抑制[³H]胸腺嘧啶核苷掺入DNA，并引起特征性形态变化。然而，AMP并未增加细胞内环磷酸腺苷的含量。胞苷一磷酸（CMP）、鸟苷一磷酸（GMP）和尿苷一磷酸（UMP）对[³H]胸腺嘧啶核苷掺入DNA有显著抑制作用，但抑制程度远小于AMP。这些核苷酸不改变细胞形态。
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