Department of Internal Medicine, Division of Cardiology, University Hospital Rostock, Germany.
Cardiovasc Diabetol. 2011 Nov 25;10:107. doi: 10.1186/1475-2840-10-107.
Bone marrow-derived circulating progenitor cells (BM-CPCs) in patients with coronary heart disease are impaired with respect to number and mobilization. However, it is unknown whether the mobilization of BM-CPCs depends on the number of diseased coronary arteries. Therefore, in our study, we analysed the correlation between the diseased coronary arteries and the frequency of CD34/45+ BM-CPCs in peripheral blood (PB) in patients with ischemic heart disease (IHD).
The frequency of CD34/45+ BM-CPCs was measured by flow cytometry in 120 patients with coronary 1 vessel (IHD1, n = 40), coronary 2 vessel (IHD2, n = 40), coronary 3 vessel disease (IHD3, n = 40) and in a control group of healthy subjects (n = 40). There was no significant difference of the total number of cardiovascular risk factors between IHD groups, beside diabetes mellitus (DM), which was significantly higher in IHD3 group compared to IHD2 and IHD1 groups.
The frequency of CD34/45+ BM-CPCs was significantly reduced in patients with IHD compared to the control group (CD34/45+; p < 0.001). The frequency of BM-CPCs was impaired in patients with IHD3 compared to IHD1 (CD34/45+; p < 0.001) and to IHD2 (CD34/45+; p = 0.001). But there was no significant difference in frequency of BM-CPCs between the patients with IHD2 and IHD1 (CD34/45+; p = 0.28). In a subgroup we observed a significant negative correlation between levels of hemoglobin AIc (HbAIc) and the frequency of BM-CPCs (CD34/45+; p < 0.001, r = -0.8).
The frequency of CD34/45+ BM-CPCs in PB is impaired in patients with IHD. This impairment may augment with an increased number of diseased coronary arteries. Moreover, the frequency of CD34/45+ BM-CPCs in ischemic tissue is further impaired by diabetes in patients with IHD.
冠心病患者骨髓来源的循环祖细胞（BM-CPCs）在数量和动员方面存在受损。然而，目前尚不清楚 BM-CPCs 的动员是否取决于患病冠状动脉的数量。因此，在本研究中，我们分析了缺血性心脏病（IHD）患者外周血（PB）中 CD34/45+ BM-CPCs 的频率与患病冠状动脉之间的相关性。
通过流式细胞术测量 120 例单支病变（IHD1，n=40）、双支病变（IHD2，n=40）、三支病变（IHD3，n=40）冠心病患者和 40 例健康对照组中 CD34/45+ BM-CPCs 的频率。除糖尿病（DM）外，IHD 组的心血管危险因素总数无显著差异，而 IHD3 组的 DM 明显高于 IHD2 和 IHD1 组。
与对照组相比，IHD 患者的 CD34/45+ BM-CPCs 频率显著降低（CD34/45+；p<0.001）。与 IHD1 相比，IHD3 患者的 BM-CPCs 频率受损（CD34/45+；p<0.001），与 IHD2 相比（CD34/45+；p=0.001）。然而，IHD2 和 IHD1 患者的 BM-CPCs 频率无显著差异（CD34/45+；p=0.28）。在一个亚组中，我们观察到 HbAIc（HbAIc）水平与 BM-CPCs 频率之间存在显著负相关（CD34/45+；p<0.001，r=-0.8）。
IHD 患者 PB 中 CD34/45+ BM-CPCs 的频率受损。这种损伤可能随着患病冠状动脉数量的增加而增加。此外，在 IHD 患者中，缺血组织中 CD34/45+ BM-CPCs 的频率进一步受到糖尿病的损害。
