Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea.
Fish Shellfish Immunol. 2012 Jan;32(1):94-100. doi: 10.1016/j.fsi.2011.10.027. Epub 2011 Nov 17.
The effect of Withania somnifera extract supplementation diets on innate immune response in giant freshwater prawn Macrobrachium rosenbergii (de Man) against Aeromonas hydrophila was investigated. The bacterial clearance efficiency significantly increased in prawn fed with 0.1% and 1.0% doses of W. somnifera supplementation diet against pathogen from weeks 1-4 as compared to the control. The innate immune parameters such as, phenoloxidase activity, superoxide anion level, superoxide dismutase activity, nitrate, and nitrite concentrations were significantly enhanced in prawn fed with 0.1% and 1.0% doses of W. somnifera supplementation diet from weeks 1-4 against pathogen. The total hemocyte counts (THC) significantly increased in prawn fed with 0.1% and 1.0% doses diet from weeks 1-4 against pathogen as compared to the control. These results strongly suggested that administration of W. somnifera through supplementation diet positively enhances the innate immune system and enhanced survival rate in M. rosenbergii against A. hydrophila infection.
研究了睡茄提取物补充饮食对巨型淡水虾Macrobrachium rosenbergii（de Man）对嗜水气单胞菌固有免疫反应的影响。与对照组相比，从第 1 周到第 4 周，喂食 0.1%和 1.0%剂量的睡茄补充饮食的虾的细菌清除效率显著提高。从第 1 周到第 4 周，喂食 0.1%和 1.0%剂量的睡茄补充饮食的虾的酚氧化酶活性、超氧阴离子水平、超氧化物歧化酶活性、硝酸盐和亚硝酸盐浓度等固有免疫参数均显著增强。与对照组相比，从第 1 周到第 4 周，喂食 0.1%和 1.0%剂量饮食的虾的总血细胞计数（THC）均显著增加。这些结果强烈表明，通过补充饮食给予睡茄可积极增强虾的免疫系统，并提高其对嗜水气单胞菌感染的存活率。
