Université de Corse, UMR-CNRS 6134, Laboratoire Chimie des Produits Naturels, Campus Grimaldi, BP 52, 20250 Corte, France.
Phytochemistry. 2012 Feb;74:123-32. doi: 10.1016/j.phytochem.2011.09.002. Epub 2011 Nov 24.
Chemical and genetic diversity of Teucrium scorodonia L. subsp. scorodonia from Corsica and T. scorodonia L. subsp. baeticum from western Algeria were investigated. Diversity within and among the two populations of subspecies was assessed according to the chemical composition of their essential oils, and genetic diversity was evaluated using three polymorphic genetic markers. Chemical analysis was performed using a combination of capillary GC-RI and GC/MS after fractionation using column chromatography. Genetic structures were mapped using two chloroplast markers (RPL32-TRNL and TRNL-F) and ribosomal nuclear markers (ITS region). The statistical analysis showed that the two subspecies were clearly distinguished by these chemical and genetic markers. The chemical composition of oil differed qualitatively and quantitatively between the subspecies. Corsican oil samples contained germacrene B (4.2-8.8%) and γ-elemene (2.6-5.7%), which were not detected in Algerian oil samples. The oils of the scorodonia and baeticum subspecies were dominated by sesquiterpene hydrocarbon compounds (75.6-82.9% and 69.6-79.4%, respectively), but they differed in oxygenated sesquiterpene content (3.1-8.9% and 8.4-20.3%, respectively). Neighbor-joining trees constructed from chloroplast DNA and ITS region sequences showed the existence of two groups associated with taxonomic and chemical characteristics. One group consisted of T. scorodonia subsp. scorodonia and the other of T. scorodonia subsp. baeticum, indicating that variation in the essential oil composition of T. scorodonia subspecies depends more on genetic background than environmental characteristics.
对来自科西嘉岛的 Teucrium scorodonia L. 亚种 scorodonia 和来自阿尔及利亚西部的 T. scorodonia L. 亚种 baeticum 的化学和遗传多样性进行了研究。根据其精油的化学成分评估了两个亚种群体内和群体间的多样性，并使用三个多态性遗传标记评估了遗传多样性。化学分析使用毛细管 GC-RI 和 GC/MS 组合在使用柱色谱进行馏分后进行。使用两个叶绿体标记（RPL32-TRNL 和 TRNL-F）和核核糖体标记（ITS 区）绘制遗传结构。统计分析表明，这两个亚种可以通过这些化学和遗传标记清楚地区分。油的化学成分在亚种之间存在定性和定量差异。科西嘉岛的油样含有反式-大根香叶烯 B（4.2-8.8％）和γ-榄香烯（2.6-5.7％），而在阿尔及利亚油样中未检测到。 scorodonia 和 baeticum 亚种的油主要由倍半萜烃化合物组成（分别为 75.6-82.9％和 69.6-79.4％），但含氧倍半萜含量不同（分别为 3.1-8.9％和 8.4-20.3％）。基于叶绿体 DNA 和 ITS 区序列构建的邻接树显示存在与分类和化学特征相关的两个组。一组由 T. scorodonia 亚种 scorodonia 组成，另一组由 T. scorodonia 亚种 baeticum 组成，表明 T. scorodonia 亚种精油成分的变化更多地取决于遗传背景而不是环境特征。
