Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskişehir 26470, Turkey.
Chem Biodivers. 2012 Jun;9(6):1144-54. doi: 10.1002/cbdv.201100264.
In the present work, three varieties of Teucrium orientale, var. orientale, var. puberulens, and var. glabrescens, were collected and investigated for chemical composition of the oils. Subsequent gas chromatography (GC-FID) and gas chromatography coupled to mass spectrometry (GC/MS) revealed high abundance of sesquiterpenes in the essential oils analyzed. All the oils contained β-caryophyllene (22.6, 8.5, and 6.3%, resp.) and hexadecanoic acid (7.9, 12.8, and 13.1%). Germacrene D (24.6 and 33.4%) and bicyclogermacrene (6.7 and 8.5%) were found to be the main constituents of var. orientale and var. puberulens, respectively. The high percentages of β-cubebene (26.9%), α-cubebene (9.0%), and α-copaene (7.2%) established the diversity of var. glabrescens. The qualitative difference between the essential oils allowed the differentiation between the varieties in agreement with the morphological observations described in Flora of Turkey for each variety studied. In addition, a cluster analysis of twelve Teucrium taxa based on the essential-oil composition has been carried out. Hovewer, the analysis did not clearly reflect the infrageneric classification of the genus, it largely confirmed the relationships between the infraspecific taxa of Teucrium orientale and T. chamaedrys.
在本工作中，采集并研究了东方筋骨草的三个变种，即东方变种、多毛变种和无毛变种，以研究其油的化学成分。随后的气相色谱（GC-FID）和气相色谱-质谱联用（GC/MS）分析表明，这些精油中含有大量的倍半萜。所有的油都含有β-石竹烯（分别为 22.6%、8.5%和 6.3%）和十六烷酸（分别为 7.9%、12.8%和 13.1%）。发现 germacrene D（24.6% 和 33.4%）和双环 germacrene（6.7% 和 8.5%）分别是东方变种和多毛变种的主要成分。β-荜澄茄烯（26.9%）、α-荜澄茄烯（9.0%）和α-罗勒烯（7.2%）的高含量确立了无毛变种的多样性。精油的定性差异允许根据每种研究变种的《土耳其植物志》中描述的形态观察对品种进行区分。此外，还根据精油成分对 12 种筋骨草进行了聚类分析。然而，该分析并没有清楚地反映属的种下分类，它在很大程度上证实了东方筋骨草和 chamaedrys 筋骨草的种下分类群之间的关系。
