Division of Molecular Biology, Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan.
Biochem Biophys Res Commun. 2011 Dec 9;416(1-2):217-21. doi: 10.1016/j.bbrc.2011.11.040. Epub 2011 Nov 15.
We have previously reported that only a single Cdk1-related G1 and G1/S cyclin homologue was found in the genome sequence of the pathogenic basidiomycetous yeast Cryptococcus neoformans (C. neoformans) and designated it CnCln1. Surprisingly, CnCln1 was not only able to complement the function of the G1 cyclins of the ascomycetous budding yeast Saccharomyces cerevisiae (S. cerevisiae), such as ScCln3, but also the G1/S cyclins of S. cerevisiae, such as ScCln1 and ScCln2. In this study, we investigated how CnCln1 cooperates with the cyclin-dependent kinases of S. cerevisiae (ScCdk1) and substitutes the function of G1 and G1/S cyclins of S. cerevisia from a point of view of their structure-function relationship. Our in silico analysis demonstrated that the CnCln1/ScCdk1 complex was more stable than any of the yeast cyclin and ScCdk1complexes. Thus, these results are consistent with in vitro analysis that has revealed the flexible functional capacity of CnCln1 as a Cdk1-related G1 and G1/S cyclins of S. cerevisiae.
我们之前曾报道过，致病性担子菌新生隐球菌（C. neoformans）的基因组序列中仅发现了一个与 Cdk1 相关的 G1 和 G1/S 细胞周期蛋白同源物，并将其命名为 CnCln1。令人惊讶的是，CnCln1 不仅能够补充酿酒酵母（S. cerevisiae）中 G1 细胞周期蛋白（如 ScCln3）的功能，还能够补充 S. cerevisiae 中的 G1/S 细胞周期蛋白，如 ScCln1 和 ScCln2。在这项研究中，我们从结构-功能关系的角度研究了 CnCln1 如何与酿酒酵母（ScCdk1）的细胞周期蛋白依赖性激酶（ScCdk1）合作，并替代酿酒酵母的 G1 和 G1/S 细胞周期蛋白的功能。我们的计算机分析表明，CnCln1/ScCdk1 复合物比任何酵母细胞周期蛋白和 ScCdk1 复合物都更稳定。因此，这些结果与体外分析一致，表明 CnCln1 具有作为酿酒酵母 Cdk1 相关 G1 和 G1/S 细胞周期蛋白的灵活功能能力。
