采用高效液相色谱法同时测定香烟烟雾冷凝物中的氨基-α-咔啉和氨基-γ-咔啉。
Simultaneous determination of amino-alpha-carbolines and amino-gamma-carbolines in cigarette smoke condensate by high-performance liquid chromatography.
作者信息
Manabe S, Wada O, Kanai Y
机构信息
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
出版信息
J Chromatogr. 1990 Jul 13;529(1):125-33. doi: 10.1016/s0378-4347(00)83813-x.
A method for the simultaneous detection of amino-alpha-carbolines (2-amino-alpha-carboline and 2-amino-3-methyl-alpha-carboline) and amino-gamma-carbolines (3-amino-1,4-dimethyl-5H-pyrido [4,3-b]indole and 3-amino-1-methyl-5H-pyrido [4,3-b]indole) by high-performance liquid chromatography has been developed. It consists of a three-step purification using three different columns with fluorometric detection. With this method, we have demonstrated that both amino-alpha-carbolines and amino-gamma-carbolines are present in cigarette smoke condensate. The method may be useful for detecting these carcinogens in various materials.
已开发出一种通过高效液相色谱同时检测氨基-α-咔啉（2-氨基-α-咔啉和2-氨基-3-甲基-α-咔啉）和氨基-γ-咔啉（3-氨基-1,4-二甲基-5H-吡啶并[4,3-b]吲哚和3-氨基-1-甲基-5H-吡啶并[4,3-b]吲哚）的方法。该方法包括使用三根不同的柱子进行三步纯化，并采用荧光检测。通过这种方法，我们已证明氨基-α-咔啉和氨基-γ-咔啉均存在于香烟烟雾冷凝物中。该方法可能有助于检测各种材料中的这些致癌物。
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