Department of Orthopaedic Surgery, Yamagata University School of Medicine, Yamagata, Japan.
Arthroscopy. 2012 Feb;28(2):255-63. doi: 10.1016/j.arthro.2011.08.294. Epub 2011 Nov 25.
The purpose of this study was to investigate the in vivo effects of gelatin hydrogels (GHs) incorporating fibroblast growth factor 2 (FGF-2) on meniscus repair in a rabbit model.
FGF-2 was biologically stabilized by incorporation into GHs. This system enables FGF-2 to be released with its biologic activity intact. A total of 64 skeletally mature female Japanese white rabbits were used. A horizontal tear was made in the medial meniscus, and these tears were divided into 4 groups: GH-FGF, GH-no FGF, FGF (FGF-2 alone), and no treatment. The meniscus was evaluated histologically at 2, 4, 8, and 12 weeks after surgery. Cell density and the percentages of proliferating cell nuclear antigen-positive cells and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling-positive cells were measured, and a scoring system ranging from 5 points (complete healing) to 0 points (no evidence of healing) was used.
Cell density was significantly higher in the GH-FGF group than in the other 3 groups at 2, 4, 8, and 12 weeks (P < .01). The percentage of proliferating cell nuclear antigen-positive cells was significantly higher whereas the percentage of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling-positive cells was significantly lower in the GH-FGF group at 2 and 4 weeks after surgery (P < .05). At 4, 8, and 12 weeks after surgery, healing scores were significantly higher in the GH-FGF group (2.5 points, 2.7 points, and 3.0 points, respectively) than in the GH-no FGF group (1.3 points, 1.4 points, and 2.0 points, respectively) (P < .05).
GHs incorporating FGF-2 significantly stimulated proliferation and inhibited the death of meniscal cells until 4 weeks, thereby increasing meniscal cell density and enhancing meniscal repair in a rabbit model.
GHs incorporating FGF-2 are able to enhance the healing of meniscal injury.
本研究旨在探讨在兔模型中，含有成纤维细胞生长因子 2（FGF-2）的明胶水凝胶（GHs）对半月板修复的体内作用。
通过将 FGF-2 生物稳定地掺入 GHs 中，使 FGF-2 能够完整地释放其生物活性。总共使用 64 只成熟的雌性日本白兔。在半月板内侧造成水平撕裂，将这些撕裂分为 4 组：GH-FGF、GH-无 FGF、FGF（FGF-2 单独）和无治疗。手术后 2、4、8 和 12 周对半月板进行组织学评估。测量细胞密度以及增殖细胞核抗原阳性细胞和末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记阳性细胞的百分比，并使用从 5 分（完全愈合）到 0 分（无愈合证据）的评分系统。
在 2、4、8 和 12 周时，GH-FGF 组的细胞密度明显高于其他 3 组（P <.01）。在手术后 2 和 4 周时，GH-FGF 组增殖细胞核抗原阳性细胞的百分比明显较高，而末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记阳性细胞的百分比明显较低（P <.05）。手术后 4、8 和 12 周时，GH-FGF 组的愈合评分（分别为 2.5 分、2.7 分和 3.0 分）明显高于 GH-无 FGF 组（分别为 1.3 分、1.4 分和 2.0 分）（P <.05）。
含有 FGF-2 的 GHs 显著刺激了半月板细胞的增殖并抑制了其死亡，直到 4 周，从而增加了半月板细胞密度，并增强了兔模型中的半月板修复。
含有 FGF-2 的 GHs 能够增强半月板损伤的愈合。
