Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, PR China.
J Hazard Mater. 2012 Jan 15;199-200:309-13. doi: 10.1016/j.jhazmat.2011.11.008. Epub 2011 Nov 9.
Transgenic Arabidopsis thaliana were developed to increase tolerance for and accumulation of heavy metals and metalloids by simultaneous overexpression of AsPCS1 and YCF1 (derived from garlic and baker's yeast) based on the fact that chelation of metals and vacuolar compartmentalization are the main strategies for heavy metals/metalloids detoxification and tolerance in plants. Dual-gene transgenic lines had the longest roots and the highest accumulation of Cd and As than single-gene transgenic lines and wildtype. When grown on cadmium or arsenic (arsenite/arsenate), Dual-gene transgenic lines accumulated over 2-10 folds cadmium/arsenite and 2-3 folds arsenate than wild type or plants expressing AsPCS1 or YCF1 alone. Such stacking modified genes involved in chelation of toxic metals and vacuolar compartmentalization represents a highly promising new tool for use in phytoremediation efforts.
为提高拟南芥对重金属和类金属的耐受性和积累能力，我们基于螯合金属和液泡区隔化是植物体内重金属/类金属解毒和耐受的主要策略，通过同时过表达大蒜和面包酵母来源的 AsPCS1 和 YCF1，构建了转基因拟南芥。与单基因转化系和野生型相比，双基因转化系的根最长，Cd 和 As 的积累量最高。在 Cd 或 As（亚砷酸盐/砷酸盐）存在的情况下，双基因转化系积累的 Cd/亚砷酸盐是野生型或单独表达 AsPCS1 或 YCF1 植物的 2-10 倍，而砷酸盐是野生型或单独表达 AsPCS1 或 YCF1 植物的 2-3 倍。这种叠加修饰参与螯合有毒金属和液泡区隔化的基因，代表了在植物修复工作中极具应用前景的新工具。
