Animal Genetic Improvement Division, National Institute of Animal Science, Seonghwan, Cheonan 333801, Republic of Korea.
Meat Sci. 2012 Mar;90(3):711-4. doi: 10.1016/j.meatsci.2011.10.017. Epub 2011 Nov 4.
This study was designed to investigate the effects of calpastatin genotypes determined by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) on calpastatin activity (CAC) and Warner-Bratzler Shear Force (WBS). Longissimus muscles were prepared from 379 Hanwoo bulls aged approximately 20months. The selection of PCR primers was based on exons (27 and 28) of the bovine calpastatin cDNA sequences, and genetic variants were detected by SSCP analysis using Taq I restriction enzymes. Sequencing analysis confirmed 4 restriction sites (nucleotide positions 52, 67, 796, and 1369), and a genetic variant was verified at a nucleotide position 641 (C/T substitutions) based on sequences (AF281256). The CAST28 genotypes showing allele frequencies of C (0.429) and T (0.571) were significantly associated with CAC and WBS. A significant positive residual correlation (r=0.121, P=0.02) between CAC and WBS was obtained.
本研究旨在通过聚合酶链反应-单链构象多态性（PCR-SSCP）分析钙蛋白酶抑制蛋白（CAST）基因型对钙蛋白酶活性（CAC）和 Warner-Bratzler 剪切力（WBS）的影响。从约 20 月龄的 379 头韩牛公牛中提取背最长肌。PCR 引物的选择基于牛钙蛋白酶抑制蛋白 cDNA 序列的外显子（27 和 28），并通过 Taq I 限制酶的 SSCP 分析检测遗传变异。测序分析证实了 4 个限制位点（核苷酸位置 52、67、796 和 1369），并根据序列（AF281256）在核苷酸位置 641（C/T 取代）验证了一个遗传变异。等位基因频率为 C（0.429）和 T（0.571）的 CAST28 基因型与 CAC 和 WBS 显著相关。CAC 和 WBS 之间存在显著的正残差相关性（r=0.121，P=0.02）。
