Csabai Judit, Nagy Z, Mándy Aandrea T
Nyíregyháza Botanical Garden, Sóstói út 31/b H-4400 Nyíregyháza, Hungary.
Acta Biol Hung. 2011 Dec;62(4):453-62. doi: 10.1556/ABiol.62.2011.4.10.
In vitro shoot multiplication of Telekia speciosa (Schreb.) Baumg. was tested on media containing benzyladenine, benzyladenine riboside, kinetin, zeatin, meta-topolin or 2-isopentenyladenine in different concentrations. We observed the proliferation rate, the length of shoots, rate of callus formation, and the presence of the hyperhydricity. The highest proliferation rate (13.17) was obtained on medium supplemented with 5.0 μM benzyladenine, however, the leaves were hyperhydrated at this concentration of benzyladenine, therefore for shoot multiplication lower (1.0 μM BA) concentration of benzyladenine is suggested. The longest shoots were achieved using 1.0 μM 2-iP. At this treatment 100% rooting was found, therefore the stage of rooting is omissible using 1.0 μM 2-iP during the multiplication. This in vitro propagation protocol should be useful for conservation as well as mass propagation of this plant species.
在含有不同浓度苄基腺嘌呤、苄基腺嘌呤核苷、激动素、玉米素、间-拓扑替康或2-异戊烯基腺嘌呤的培养基上，对美丽独活（Telekia speciosa (Schreb.) Baumg.）进行了离体芽增殖试验。我们观察了增殖率、芽的长度、愈伤组织形成率以及玻璃化现象的出现情况。在添加5.0 μM苄基腺嘌呤的培养基上获得了最高增殖率（13.17），然而，在此苄基腺嘌呤浓度下叶片出现了玻璃化现象，因此建议在芽增殖时使用较低浓度（1.0 μM BA）的苄基腺嘌呤。使用1.0 μM 2-异戊烯基腺嘌呤获得了最长的芽。在此处理下发现生根率为100%，因此在增殖过程中使用1.0 μM 2-异戊烯基腺嘌呤时可省略生根阶段。这种离体繁殖方案对于该植物物种的保存和大规模繁殖应该是有用的。
