Department of Botany, MMV, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
Appl Biochem Biotechnol. 2013 Apr;169(8):2303-14. doi: 10.1007/s12010-013-0157-7. Epub 2013 Feb 28.
An efficient, rapid, and highly reproducible regeneration protocol was successfully developed for Phyllanthus fraternus from the field-derived mature nodal segments. The explants induced multiple shoots on cytokinin containing medium. The highest frequency (99 %) and maximum number of shoots (19.75) were induced on Murashige and Skoog's (MS) medium supplemented with 2.22 μM 6-benzylaminopurine after 3-4 weeks of culture initiation. The elongated shoots were rooted on MS medium supplemented with indol-3-butyric acid (IBA) or α-naphthalene acetic acid. Pulse treatment of microshoots promoted significant increase in the percentage of rooting and number of root regeneration per shoot. The highest rooting (100 %) and maximum number of roots (8.75) per shoot was obtained when shoots were dipped in IBA solution (0.98 mM) for 5 min and further subcultured on MS basal medium. Plantlets were successfully acclimatized and established in soil. Regenerated plants were grown normally in the field without showing any morphological variations. This cost-effective protocol will help the mass multiplication of P. fraternus for commercial propagation and high biomass production of this valuable medicinal plant.
从野外成熟的节段成功开发出一种高效、快速且高度可重复的叶下珠再生方案。外植体在含细胞分裂素的培养基上诱导出多个芽。在含有 2.22 μM 6-苄氨基嘌呤的 Murashige 和 Skoog 培养基上培养 3-4 周后，诱导出的芽频率最高（99%），芽数量最多（19.75）。伸长的芽在添加吲哚丁酸（IBA）或α-萘乙酸的 MS 培养基上生根。微芽的脉冲处理可显著提高生根率和每芽的生根数。当芽在 IBA 溶液（0.98 mM）中浸泡 5 分钟并进一步在 MS 基本培养基上培养时，可获得最高的生根率（100%）和每芽最大根数量（8.75）。组培苗成功适应并在土壤中建立。再生植物在田间正常生长，没有表现出任何形态变异。这种具有成本效益的方案将有助于叶下珠的大规模繁殖，用于商业推广和这种有价值的药用植物的高生物量生产。
