Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, Universités de Provence et de la Méditerranée, ESIL Case 925, 163 Avenue de Luminy, Marseille Cedex 9, France.
Antonie Van Leeuwenhoek. 2012 Mar;101(3):595-602. doi: 10.1007/s10482-011-9675-x. Epub 2011 Nov 26.
A novel strictly anaerobic bacterium designated SPDX02-08(T) was isolated from a deep terrestrial geothermal spring located in southwest France. Cells (1-2 × 2-6 μm) were non-motile, non sporulating and stained Gram negative. Strain SPDX02-08(T) grew at a temperature between 40 and 60°C (optimum 55°C), pH between 6.3 and 7.3 (optimum 7.2) and a NaCl concentration between 0 and 5 g/l (optimum 2 g/l). Sulfate, thiosulfate and sulfite were used as terminal electron acceptors, but not elemental sulfur, nitrate, nitrite, Fe (III) or fumarate. In the presence of sulfate, strain SPDX02-08(T) completely oxidized pyruvate, propionate, butyrate, isobutyrate, valerate, isovalerate and hexadecanoate. Stoichiometric measurements revealed a complete oxidation of part of lactate (0.125 mol of acetate produced per mole lactate oxidized). Strain SPDX02-08(T) required yeast extract to oxidize formate and H(2) but did not grow autotrophically on H(2). Among the substrates tested, only pyruvate was fermented. The G+C content of the genomic DNA was 57.6 mol%. Major cellular fatty acids of strain SPDX02-08(T) were iso-C(15:0), C(15:0), and C(16:0). Phylogenetic analysis of the 16S small-subunit (SSU) ribosomal RNA gene sequence indicated that strain SPDX02-08(T) belongs to the genus Desulfosoma, family Syntrophobacteraceae, having Desulfosoma caldarium as its closest phylogenetic relative (97.6% similarity). The mean DNA/DNA reassociation value between strain SPDX02-08(T) and Desulfosoma caldarium was 16.9 ± 2.7%. Based on the polyphasic differences, strain SPDX02-08(T) is proposed to be assigned as a new species of the genus Desulfosoma, Desulfosoma profundi sp. nov. (DSM 22937(T) = JCM 16410(T)). GenBank accession number for the 16S rRNA gene sequence of strain SPDX02-08(T) is HM056226.
从法国西南部一个深陆地温泉中分离到一株新型严格厌氧细菌，命名为 SPDX02-08(T)。细胞（1-2×2-6μm）不运动，不产芽孢，革兰氏阴性。SPDX02-08(T)株在 40-60°C（最佳 55°C）、pH 6.3-7.3（最佳 7.2）和 0-5g/l NaCl（最佳 2g/l）下生长。硫酸盐、硫代硫酸盐和亚硫酸盐可用作末端电子受体，但元素硫、硝酸盐、亚硝酸盐、Fe(III)或延胡索酸盐不可用。在硫酸盐存在下，SPDX02-08(T)株完全氧化丙酮酸、丙酸盐、丁酸盐、异丁酸盐、戊酸盐、异戊酸盐和十六烷酸盐。化学计量测量显示部分乳酸盐完全氧化（每氧化 1 摩尔乳酸盐产生 0.125 摩尔乙酸盐）。SPDX02-08(T)株需要酵母提取物才能氧化甲酸盐和 H2，但不能自养生长在 H2 上。在所测试的底物中，只有丙酮酸可被发酵。基因组 DNA 的 G+C 含量为 57.6mol%。SPDX02-08(T)株的主要细胞脂肪酸为 iso-C(15:0)、C(15:0)和 C(16:0)。16S 小亚基（SSU）核糖体 RNA 基因序列的系统发育分析表明，SPDX02-08(T)株属于脱硫弧菌属，脱硫弧菌科，与脱硫弧菌属亲缘关系最近（相似度 97.6%）。SPDX02-08(T)株与脱硫弧菌属之间的平均 DNA/DNA 再结合值为 16.9±2.7%。基于多相差异，建议将 SP DX02-08(T)株分配为脱硫弧菌属的一个新种，命名为深脱硫弧菌（Desulfosoma profundi sp. nov.）DSM 22937(T)=JCM 16410(T)。SPDX02-08(T)株 16S rRNA 基因序列的 GenBank 登录号为 HM056226。
