IRD, UMR 180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, 163 Avenue de Luminy, F-13288 Marseille cedex 9, France.
Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):3100-4. doi: 10.1099/ijs.0.010363-0. Epub 2009 Jul 30.
A novel anaerobic, chemo-organotrophic bacterium, designated VNs36(T), was isolated from a well that collected water from a deep saline aquifer used for underground gas storage at a depth of 830 m in the Paris Basin, France. Cells were curved motile rods or vibrios (3.0-5.0x0.5 microm). Strain VNs36(T) grew at temperatures between 20 and 50 degrees C (optimum 37 degrees C) and at pH values between 5.0 and 9.0 (optimum 6.9). It did not require salt for growth, but tolerated up to 20 g NaCl l(-1) (optimum 2 g l(-1)). In the presence of sulfate, strain VNs36(T) used lactate, formate and pyruvate as carbon and energy sources. The main fermentation products from lactate were acetate, H(2) and CO(2). Sulfate, thiosulfate and sulfite were used as electron acceptors, but not sulfur. The genomic DNA G+C content of strain VNs36(T) was 67.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain VNs36(T) was affiliated with the family Desulfovibrionaceae within the class Deltaproteobacteria. On the basis of 16S rRNA gene sequence comparisons, DNA G+C content and the absence of desulfoviridin in cell extracts, it is proposed that strain VNs36(T) be assigned to a new genus, Desulfocurvus gen. nov., as a representative of a novel species, Desulfocurvus vexinensis sp. nov. The type species of this genus is Desulfocurvus vexinensis with the type strain VNs36(T) (=DSM 17965(T)=JCM 14038(T)).
一种新型的厌氧、化能有机营养细菌，被命名为 VNs36(T)，是从法国巴黎盆地一个深度为 830 米的用于地下储气的深部咸水含水层的一口井中分离出来的。细胞呈弯曲的运动杆状或弧菌（3.0-5.0x0.5 微米）。VN36(T) 菌株可在 20 至 50 摄氏度（最佳 37 摄氏度）和 pH 值在 5.0 至 9.0 之间（最佳 6.9）生长。它的生长不需要盐，但可以耐受高达 20 g NaCl l(-1)（最佳 2 g l(-1)）。在硫酸盐存在的情况下，VN36(T) 菌株使用乳酸盐、甲酸盐和丙酮酸盐作为碳和能源来源。从乳酸盐中产生的主要发酵产物是乙酸盐、H(2)和 CO(2)。硫酸盐、硫代硫酸盐和亚硫酸盐被用作电子受体，但不是硫。VN36(T) 菌株的基因组 DNA G+C 含量为 67.2 mol%。16S rRNA 基因序列的系统发育分析表明，VN36(T) 菌株属于δ变形菌纲的脱硫弧菌科。根据 16S rRNA 基因序列比较、DNA G+C 含量和细胞提取物中不存在脱硫菌素，建议将 VN36(T) 菌株分配到一个新属，即 Desulfocurvus 属，作为一种新型物种 Desulfocurvus vexinensis 的代表。该属的模式种是 Desulfocurvus vexinensis，其模式菌株为 VN36(T)=DSM 17965(T)=JCM 14038(T)。
