Qi Yongfu, Wang Xuexi, Xu Zhong, Xu Xinwen, Li Shang, Zhao Jianxiong
Institute of Combined Traditional Chinese and Western Medicine, Lanzhou University, Lanzhou 730000, China.
Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(17):2418-22.
To study the influence of SGHWJN particles on inflammation and the mediators of inflammation in esophageal tissues of rat with reflux esophagitis.
Fifty SD rats were randomly divided into 5 groups, namely, a control group, a sham-operated group, a model group, a SGHWJN particles group and a PPI group. Reflux esophagitis was induced by adopting partial pyloric ligation plus cardiomyotomy. One week later, the rats were orally administered twice daily for 28 days. Pathological changes of esophagus mucous membrane were evaluated by using HE staining and Harry S. Cooper's method in every groups. MDA and SOD contents in esophageal tissues were measured by colorimetric method. Expression of TNF-alpha in esophageal tissues were examined by real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-FQ-PCR) with SYBR Green.
Model group, esophageal inflammation scores, expression of TNF-alpha in esophageal tissues and MDA contents compared with the normal group and sham operation group were significantly higher (P < 0.05). SOD contents in the esophageal tissues of the model group was significantly lower than that of control group and sham-operated group (P < 0.05). SGHWJN particles group and PPI group of esophageal tissue inflammation scores, expression of TNF-a in esophageal tissues and MDA levels than those in model group decreased significantly (P < 0.05). SOD content was significantly higher than that of model group (P < 0.05), SGHWJN particles group and PPI group showed no statistically significant difference between the above-mentioned indicators. The above-mentioned indicators showed no statistically significant difference between the normal group and sham-operated group. MDA content and expression of TNF-alpha in esophageal tissue was positively correlated with inflammatory scores of model group (r = 0.813). Model group esophageal tissue SOD content and inflammation scores were negatively correlated (r = -0.847). Esophageal tissue SOD levels were negatively correlated with MDA levels (r = -0.863).
SGHWJN particles can effectively inhibit inflammation in rat with reflux esophagitis through regulating TNF-alpha, SOD and MDA.
研究疏肝和胃降逆颗粒对反流性食管炎大鼠食管组织炎症及炎症介质的影响。
将50只SD大鼠随机分为5组，即对照组、假手术组、模型组、疏肝和胃降逆颗粒组和质子泵抑制剂（PPI）组。采用部分幽门结扎加贲门肌层切开术诱导反流性食管炎。1周后，大鼠每日灌胃2次，共28天。采用苏木精-伊红（HE）染色和Harry S. Cooper法评估各组食管黏膜的病理变化。采用比色法测定食管组织中丙二醛（MDA）和超氧化物歧化酶（SOD）含量。采用SYBR Green实时荧光定量逆转录聚合酶链反应（RT-FQ-PCR）检测食管组织中肿瘤坏死因子-α（TNF-α）的表达。
模型组食管炎症评分、食管组织中TNF-α表达及MDA含量与正常组和假手术组比较，差异有统计学意义（P<0.05）。模型组食管组织SOD含量显著低于对照组和假手术组（P<0.05）。疏肝和胃降逆颗粒组和PPI组食管组织炎症评分、食管组织中TNF-α表达及MDA水平较模型组显著降低（P<0.05）。SOD含量显著高于模型组（P<0.05），疏肝和胃降逆颗粒组和PPI组上述指标比较，差异无统计学意义。正常组和假手术组上述指标比较，差异无统计学意义。模型组食管组织MDA含量和TNF-α表达与炎症评分呈正相关（r=0.813）。模型组食管组织SOD含量与炎症评分呈负相关（r=-0.847）。食管组织SOD水平与MDA水平呈负相关（r=-0.863）。
疏肝和胃降逆颗粒可通过调节TNF-α、SOD和MDA有效抑制反流性食管炎大鼠的炎症。
