School of Clinical Sciences, Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, The University of Nottingham, UK.
Cells Tissues Organs. 2012;195(6):484-94. doi: 10.1159/000329861. Epub 2011 Nov 23.
Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling.
骨髓间充质干细胞（MSCs）被广泛用作成骨细胞的细胞模型，并且可以在体外对成骨培养基（OM）做出矿化响应。然而，尚不清楚这种特性是否是间充质来源细胞所特有的。我们分析了 3 种非成骨系细胞，即 HEK293、HeLa 和 Ntera，与 MSCs 相比，它们对 OM 条件的反应。虽然 HEK293 细胞未能对 OM 条件做出反应，但 2 种癌源性系 Ntera 和 HeLa 沉积的钙磷矿物与 MSC 培养物中的矿物相当。然而，与 MSCs 不同的是，HeLa 和 Ntera 培养物在没有地塞米松的情况下也能做到这一点。这一差异得到了证实，因为骨形态发生蛋白抑制消除了 MSCs 对 OM 的反应，但对 HeLa 或 Ntera 没有影响，表明这 2 种模型可以通过一种独立于已建立的地塞米松或骨形态发生蛋白信号的机制来沉积矿物。
