一种用于分离嘌呤碱、核苷和酰脲的高效液相色谱法：应用于高等植物嘌呤分解代谢的研究

A high-performance liquid chromatography method for separation of purine bases, nucleosides and ureides: application to studies on purine catabolism in higher plants.

作者信息

Ashihara H, Yabuki N, Mitsui K

机构信息

Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan.

出版信息

J Biochem Biophys Methods. 1990 Jun;21(1):59-63. doi: 10.1016/0165-022x(90)90045-e.

DOI:10.1016/0165-022x(90)90045-e

PMID:2212466

Abstract

Mixtures of purine nucleotides, nucleosides, nucleobases, uric acid, allantoin and allantoic acid were fractionated by high-performance liquid chromatography on a polyvinyl alcohol gel column, Asahipak GS-320H, with isocratic elution by sodium phosphate. Application of this system to the determination of the sizes of cellular pools of purine derivatives in plant cells and of the activity of related enzymes, as well as to the purification of enzymatically synthesized radioactive compounds, is described.

摘要

通过在聚乙烯醇凝胶柱（Asahipak GS - 320H）上进行高效液相色谱，用磷酸钠等度洗脱，对嘌呤核苷酸、核苷、核碱基、尿酸、尿囊素和尿囊酸的混合物进行了分离。描述了该系统在测定植物细胞中嘌呤衍生物的细胞池大小和相关酶活性以及纯化酶促合成的放射性化合物方面的应用。