Division of Molecular & Life Sciences, Pohang University of Science & Technology (POSTECH), Pohang 790-784, Republic of Korea.
Epigenomics. 2011 Feb;3(1):73-81. doi: 10.2217/epi.10.72.
Cell reprogramming has been known to accompany cell type-specific epigenetic alterations of the genome. Chromatin structure and dynamics influenced by epigenetic factors such as covalent histone modifications, histone variants, DNA methylation, ncRNAs and chromatin remodeling play an important role in determining cell fate. The rapid progress made with the development of high-throughput technology and the systematic assessment of accumulated data has enabled the identification of previously unknown biological processes and disease states in terms of whole-genome profiles of epigenetic signatures at a high resolution. In this article, we discuss the fundamental advances and challenges over the past several years in our knowledge of chromatin state and gene transcription programs associated with epigenetic changes during cell reprogramming processes. In particular, histone modifications, DNA methylation and transcriptome analyses in genome-scale studies will be reviewed to characterize a functional cross-talk between epigenetic and transcriptional regulations in cell reprogramming.
细胞重编程伴随着基因组的细胞类型特异性表观遗传改变。受表观遗传因素（如共价组蛋白修饰、组蛋白变体、DNA 甲基化、ncRNAs 和染色质重塑）影响的染色质结构和动力学在决定细胞命运方面发挥着重要作用。随着高通量技术的发展和对积累数据的系统评估，我们能够以前所未有的分辨率从全基因组表观遗传特征的角度来识别以前未知的生物过程和疾病状态。在本文中，我们讨论了过去几年中在我们对与细胞重编程过程中表观遗传变化相关的染色质状态和基因转录程序的认识方面取得的重大进展和挑战。特别是，将对组蛋白修饰、DNA 甲基化和全基因组研究中的转录组分析进行综述，以描述细胞重编程中表观遗传和转录调控之间的功能串扰。
