Laboratory of Environmental Technology, Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, China.
FEMS Microbiol Ecol. 2012 Apr;80(1):9-18. doi: 10.1111/j.1574-6941.2011.01263.x. Epub 2012 Jan 11.
The physiochemical and functional diversity of soil-attached microorganisms was investigated using a stabilized laboratory-scale soil aquifer treatment (SAT) system. In this system, reclaimed water after ozonation was used as the feed water, and 60% dissolved organic carbon was removed by the unsaturated vadose layer in 0.8 days. Soil biomass (volatile solids, phospholipid extraction) and functional diversity significantly decreased from the unsaturated vadose layer to the saturated aquifer, where they maintained the same level. Using principal components analysis based on substrate utilization pattern, the vadose layer soil sample was clearly separated from the saturated layer samples. Exceptionally, the oxidation rates of esters remained stable during SAT, indicating the purification potential on certain recalcitrant organic compounds in the saturated aquifer given an adequate retention time. Correlation analysis revealed that organic carbon was the key limiting factor for microbial biomass and activity, especially for tyrosine-like aromatic proteins and soluble microbial byproduct-like materials.
采用稳定的实验室规模土壤含水层处理 (SAT) 系统研究了附着在土壤上的微生物的物理化学和功能多样性。在该系统中，臭氧处理后的再生水被用作给料水，60%的溶解有机碳在 0.8 天内被不饱和的包气带去除。土壤生物量（挥发性固体、磷脂提取）和功能多样性从不饱和包气带显著降低到饱和含水层，在那里它们保持相同的水平。基于基质利用模式的主成分分析表明，包气带土壤样品与饱和层样品明显分离。例外的是，酯的氧化速率在 SAT 期间保持稳定，表明在足够的停留时间下，饱和含水层对某些难降解有机化合物具有净化潜力。相关分析表明，有机碳是微生物生物量和活性的关键限制因素，特别是酪氨酸样芳香族蛋白质和可溶性微生物副产物样物质。
