Miller M D, Wilson S D, Dorf M E, Seuanez H N, O'Brien S J, Krangel M S
Division of Tumor Virology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
J Immunol. 1990 Oct 15;145(8):2737-44.
We previously reported the isolation and characterization of a cDNA clone, I-309, that encodes a small secreted protein produced by activated human T lymphocytes. This protein is structurally related to a large number of recently identified proteins that are secreted upon cellular activation. In this report we describe the isolation and characterization of the gene encoding I-309. The genomic organization is essentially identical to that found in the genes encoding the structurally similar proteins TCA-3, hJE/MCP-1, and mJE, strengthening the hypothesis that these genes are evolutionarily related. The region of the I-309 gene 5' of the mRNA cap site exhibits extensive nucleotide sequence homology with the same region of the murine gene TCA-3, providing additional evidence that I-309 and TCA-3 are likely to be homologs. Finally, panels of rodent-human somatic cell hybrids were used to map the I-309 gene to human chromosome 17. In conjunction with recent mapping data from other laboratories, this result suggests the presence of a cluster of related genes on this chromosome.
我们先前报道了一个cDNA克隆I-309的分离与特性鉴定，该克隆编码一种由活化的人T淋巴细胞产生的小分泌蛋白。这种蛋白在结构上与大量最近鉴定出的细胞活化时分泌的蛋白相关。在本报告中，我们描述了编码I-309的基因的分离与特性鉴定。其基因组结构与编码结构相似蛋白TCA-3、hJE/MCP-1和mJE的基因基本相同，这强化了这些基因在进化上相关的假说。I-309基因mRNA帽位点5'端区域与鼠基因TCA-3的相同区域表现出广泛的核苷酸序列同源性，提供了额外证据表明I-309和TCA-3可能是同源物。最后，利用啮齿动物-人类体细胞杂交板将I-309基因定位于人类17号染色体。结合其他实验室最近的定位数据，这一结果表明该染色体上存在一组相关基因。
