Eggers A E, Michl J
Department of Neurology, SUNY-Health Science Center, Brooklyn 11203.
J Immunol Methods. 1990 Oct 4;133(1):71-5. doi: 10.1016/0022-1759(90)90320-u.
BALB/c mice immunized with the MOPC-315 myeloma protein linked covalently to the adjuvant muramyl dipeptide developed antibodies against the MOPC protein. The anti-idiotypic nature of the antibodies was demonstrated by the ability of N epsilon-2,4-dinitrophenyl-l-lysine, a known MOPC-315 ligand, to block antibody binding. Immunized mice developed protection against in vivo challenge with MOPC-315 tumor cells. No anti-tumor cell-mediated cytotoxicity could be demonstrated in immunized and challenged mice.
用与佐剂胞壁酰二肽共价连接的MOPC - 315骨髓瘤蛋白免疫的BALB/c小鼠产生了针对MOPC蛋白的抗体。已知的MOPC - 315配体Nε - 2,4 - 二硝基苯基 - L - 赖氨酸能够阻断抗体结合，从而证明了这些抗体的抗独特型性质。免疫后的小鼠对MOPC - 315肿瘤细胞的体内攻击产生了保护作用。在免疫并受到攻击的小鼠中未显示出抗肿瘤细胞介导的细胞毒性。
