Department of Cell Biology, Histology and Pharmacology, Institute of Neurosciences of Castile and Leon, (INCYL), University of Valladolid, Spain.
Histol Histopathol. 2012 Jan;27(1):95-102. doi: 10.14670/HH-27.95.
Schwann cells (SCs) are basic elements for cell therapy and tissue engineering in the central and peripheral nervous system. Therefore, the development of a reliable method to obtain SC cultures is required. For possible therapeutic applications the cultures need to produce a sufficiently large number of SCs with a high level of purity in a relatively short period of time. To increase SC yield and purity we pre-degenerated pieces of 1-2 mm of adult rabbit sciatic nerves by incubating them for seven days in Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum, penicillin/streptomycin and NRG1-β1. Following pre-degeneration the nerve pieces were dissociated and then cultured for 6 or 15 days in the same culture medium. After 6 days of culture we obtained around 9.5x10³ cells/mg with approximately 94% SCs (S-100 positive) purity. After 15 days of culture the yield was about 80x10³ cells/mg and the purity was approximately 75%. Pre-degeneration and subsequent culture of small pieces of adult nerve with NRG1-β1 supplemented medium increased the number of SCs and restricted the overgrowth of fibroblast-like cells.
许旺细胞（SCs）是中枢和周围神经系统细胞治疗和组织工程的基本要素。因此，需要开发一种可靠的方法来获得 SC 培养物。为了可能的治疗应用，培养物需要在相对较短的时间内产生足够数量的具有高纯度的 SC。为了提高 SC 的产量和纯度，我们通过在含有 10%胎牛血清、青霉素/链霉素和 NRG1-β1 的 DMEM 中孵育 1-2 毫米成年兔坐骨神经片段 7 天来预退化。预退化后，将神经片段解离，然后在相同的培养基中培养 6 或 15 天。培养 6 天后，我们获得了约 9.5x10³个细胞/mg，纯度约为 94%（S-100 阳性）。培养 15 天后，产量约为 80x10³个细胞/mg，纯度约为 75%。NRG1-β1 补充培养基的预退化和随后的小块成年神经培养增加了 SC 的数量，并限制了成纤维样细胞的过度生长。
