Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Department of Microbiology and Parasitology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Iran J Basic Med Sci. 2015 Mar;18(3):307-11.
Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve.
Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The total cell yield and the purity of SCs were evaluated in each group on the first and seventh day after plating.
The maximum and minimum numbers of cells were counted in 15 day-long predegene-ration and control groups which underwent no predegeneration. The 10 day-long in vitro predegeneration group with 80±0.5% SCs enrichment had the best purity after plating day and could maintain its purity with elapsing on cultures.
10 day-long predegeneration results in the higher cell number and the better and prolonged purity of SCs in culture.
去神经预分化是获得有丝分裂激活和富集雪旺细胞（SCs）培养物的标准技术。本研究首次评估了不同去神经预分化时间对狗外周神经SCs 细胞产量和富集的影响。
狗的腓肠神经进行 5、10、15 天长时间体外去神经预分化。在接种后第 1 天和第 7 天，评估每组的总细胞产量和SCs 的纯度。
未经去神经预分化的 15 天和对照组的细胞数量最多和最少。10 天的体外去神经预分化组 SCs 纯度为 80±0.5%，接种后纯度最佳，并随着培养时间的延长仍能保持其纯度。
10 天的去神经预分化可增加细胞数量，并使SCs 在培养中获得更好和更长时间的纯度。
