Applied Biotechnology and the Department of Applied Genomics, Bristol-Myers Squibb Company, 311 Pennington-Rocky Hill Road, Pennington, NJ, 08534, USA.
Diabetes Ther. 2010 Dec;1(2):57-92. doi: 10.1007/s13300-010-0006-4. Epub 2010 Dec 17.
SGLT2 (for "Sodium GLucose coTransporter" protein 2) is the major protein responsible for glucose reabsorption in the kidney and its inhibition has been the focus of drug discovery efforts to treat type 2 diabetes. In order to better clarify the human tissue distribution of expression of SGLT2 and related members of this cotransporter class, we performed TaqMan™ (Applied Biosystems, Foster City, CA, USA) quantitative polymerase chain reaction (PCR) analysis of SGLT2 and other sodium/glucose transporter genes on RNAs from 72 normal tissues from three different individuals. We consistently observe that SGLT2 is highly kidney specific while SGLT5 is highly kidney abundant; SGLT1, sodium-dependent amino acid transporter (SAAT1), and SGLT4 are highly abundant in small intestine and skeletal muscle; SGLT6 is expressed in the central nervous system; and sodium myoinositol cotransporter is ubiquitously expressed across all human tissues.
SGLT2（“钠葡萄糖协同转运蛋白 2”蛋白）是肾脏中葡萄糖重吸收的主要蛋白，其抑制作用一直是治疗 2 型糖尿病药物发现努力的重点。为了更好地阐明 SGLT2 及其相关协同转运蛋白家族在人体组织中的表达分布，我们对来自三个不同个体的 72 种正常组织的 RNA 进行了 TaqMan™（Applied Biosystems，加利福尼亚州福斯特市）定量聚合酶链反应（PCR）分析，以检测 SGLT2 和其他钠/葡萄糖转运体基因的表达。我们一致观察到 SGLT2 具有高度的肾脏特异性，而 SGLT5 则在肾脏中大量存在；SGLT1、钠依赖性氨基酸转运体（SAAT1）和 SGLT4 在小肠和骨骼肌中大量存在；SGLT6 在中枢神经系统中表达；而钠肌醇协同转运蛋白在所有人体组织中广泛表达。
