Iida S, Nozawa S
Department of Obstetrics and Gynecology, School of Medicine, Saitama Medical School.
Nihon Sanka Fujinka Gakkai Zasshi. 1990 Jul;42(7):667-74.
The cytotoxicity including complement dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC) of two kinds of monoclonal antibodies (Mo-Ab), namely, MSN-1 which was obtained by means of an immunization procedure with intact SNG-II cells from human endometrial adenocarcinoma and HMST-1 obtained from hybridoma fused lymphocytes from lymph node with murine myeloma cells were studied by the 51Cr liberation test in vitro. 1) Against SNG-II, MSN-1 and HMST-1 showed significant CDC activity at 38.4 +/- 4.2% and 41.9 +/- 4.8% respectively, when a guinea pig complement was used. However, no significant CDC, action on SKG-IIIb was induced by MSN-1 or HMST-1. 2) Mo-Ab:MSN-1 and HMST-1 showed no significant liberation of 51Cr in relation to SKG-IIIb and SNG-II when using peripheral blood lymphocyte (PBL) separated by centrifugation gradient as the effector cells, and then the cytotoxicity of PBL alone against K-562 cell, SNG-II and SKG-IIIb was 40%, 9% and 2% respectively. This suggested that neither Mo-Ab had any ADCC activity and that SKG-IIIb and SNG-II cells were NK resistant cell-lines.
通过体外51Cr释放试验研究了两种单克隆抗体（Mo-Ab）的细胞毒性，包括补体依赖性细胞毒性（CDC）和抗体依赖性细胞介导的细胞毒性（ADCC）。这两种单克隆抗体分别是：MSN-1，通过用人子宫内膜腺癌完整的SNG-II细胞进行免疫程序获得；HMST-1，通过来自淋巴结的淋巴细胞与鼠骨髓瘤细胞融合的杂交瘤获得。1）针对SNG-II，当使用豚鼠补体时，MSN-1和HMST-1分别表现出显著的CDC活性，分别为38.4±4.2%和41.9±4.8%。然而，MSN-1或HMST-1对SKG-IIIb未诱导出显著的CDC作用。2）当使用通过离心梯度分离的外周血淋巴细胞（PBL）作为效应细胞时，Mo-Ab：MSN-1和HMST-1相对于SKG-IIIb和SNG-II未显示出51Cr的显著释放，然后单独的PBL对K-562细胞、SNG-II和SKG-IIIb的细胞毒性分别为40%、9%和2%。这表明两种Mo-Ab均无任何ADCC活性，并且SKG-IIIb和SNG-II细胞是NK抗性细胞系。
