Murakami F
Department of Obstetrics and Gynecology, Kurume University School of Medicine, Japan.
Nihon Sanka Fujinka Gakkai Zasshi. 1996 Feb;48(2):125-32.
A large number monoclonal antibodies (Mo-Abs) to neoplasms have been utilized as diagnostic tools, but they have not yet been fully characterized from the biological point of view. Antibody-dependent cellular cytotoxicity, complement dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC) were investigated in Mo-Abs OC125 and SH-9 (IgG1) to ovarian tumor cell lines SHIN-3, MN-1, KEN-3 and EC by MTT assay in vitro. OC125, but not SH-9 showed prominent CDC activity against SHIN-3 and MN-1 cell lines. CDC activity was induced against OC125 and SH-9, when a guinea pig complement was used. Furthermore, ADCC, activity was significant against SHIN-3, MN-1, OC125 and SH-9, but not against KEN-3 or EC. In summary, OC125 had both CDC and ADCC activity, but SH-9 did not. We concluded that the epitope on CA125 antigen varied in OC125 and SH-9, and each of epitope in Mo-Abs may have a different biofunction.
大量针对肿瘤的单克隆抗体（Mo-Abs）已被用作诊断工具，但从生物学角度来看，它们尚未得到充分表征。通过体外MTT试验，研究了针对卵巢肿瘤细胞系SHIN-3、MN-1、KEN-3和EC的Mo-Abs OC125和SH-9（IgG1）的抗体依赖性细胞毒性、补体依赖性细胞毒性（CDC）和抗体依赖性细胞介导的细胞毒性（ADCC）。OC125对SHIN-3和MN-1细胞系表现出显著的CDC活性，而SH-9则没有。当使用豚鼠补体时，OC125和SH-9均诱导出CDC活性。此外，ADCC活性对SHIN-3、MN-1、OC125和SH-9显著，但对KEN-3或EC不显著。总之，OC125同时具有CDC和ADCC活性，而SH-9则没有。我们得出结论，OC125和SH-9中CA125抗原上的表位不同，且Mo-Abs中的每个表位可能具有不同的生物学功能。
