Ida W, Kobayashi H, Naito Y
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1990 Sep;42(9):1161-7.
Human eutopic and heterotopic endometrium were cultured and epithelial cells were isolated from stromal cells by the modified method of Satyaswaroop et al. in order to investigate the cause of elevated serum CA125 levels in patients with adenomyosis. Flow cytometry (FCM) was used to analyze the relationship between cell cycle and CA125 production. 1. Microscopically, epithelial cells showed a tadpole-like appearance, while stromal cells were spindle-shaped. However, morphologically, no difference could be found between eutopic and heterotopic endometrial cells. 2. Immunocytochemical techniques (indirect enzyme immunoassay and fluorescence method) have demonstrated the presence of CA125 both on the cell membrane and in the cytoplasm of eutopic and heterotopic epithelial cells. Stromal cells were weakly stained by the fluorescence method, but not by enzyme immunoassay. 3. Double staining of CA125 and DNA was performed and analysed with FCM. Heterotopic epithelial cells produced a significantly higher amount (16 times higher) of CA125 than the eutopic epithelial cells. Stromal cells produced a small amount of CA125. These findings suggest that the elevation of serum CA125 levels in patients with adenomyosis may be due to greatly increased production of CA125 by heterotopic epithelial cells, in addition to the production of CA125 by eutopic ones.
为了研究子宫腺肌病患者血清CA125水平升高的原因，采用Satyaswaroop等人改良的方法对人在位和异位子宫内膜进行培养，并从基质细胞中分离出上皮细胞。运用流式细胞术（FCM）分析细胞周期与CA125产生之间的关系。1. 在显微镜下，上皮细胞呈蝌蚪状，而基质细胞呈纺锤形。然而，在位和异位子宫内膜细胞在形态上并无差异。2. 免疫细胞化学技术（间接酶免疫测定法和荧光法）已证实，在位和异位上皮细胞的细胞膜及细胞质中均存在CA125。基质细胞在荧光法检测中呈弱阳性染色，但在酶免疫测定法中未染色。3. 对CA125和DNA进行双重染色，并通过FCM分析。异位上皮细胞产生的CA125量显著高于在位上皮细胞（高16倍）。基质细胞产生少量CA125。这些发现表明，子宫腺肌病患者血清CA125水平升高可能是由于异位上皮细胞产生的CA125大量增加，此外在位上皮细胞也会产生CA125。
