Kobayashi H, Ida W, Terao T, Kawashima Y
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1992 Mar;44(3):303-9.
We have established an in vitro system in which epithelial cells (EC) and stromal cells (SC) from the human endometrium are placed in culture to examine the production of CA125. There was a much greater amount of CA125 in heterotopic EC after culture cells reached confluence than during the logarithmic growth phase. Heterotopic EC in culture secreted nine times as much CA125 constitutively after reaching confluence as eutopic EC. Flow cytometry (FCM) analysis indicated that the changes in CA125 expression correlated with the cell cycle. The CA125 expression was mainly observed in the G0/G1-phase in the cell cycle. There was no amplification of the CA125 expression in heterotopic EC membranes. The results of SDS-PAGE and Western blot indicated that the 110 KDa molecule of CA125 might be specific for adenomyosis. The biochemical and physical nature of CA125 was examined to characterize the antigenic determinant of this antigen. These results strongly suggested that the CA125 antigenic determinant from EC was composed of conformationally dependent peptides. We conclude that significantly increased secretion of CA125 from heterotopic EC could be attributed to the increase in serum CA125 in patients with adenomyosis.
我们建立了一个体外系统，将人子宫内膜的上皮细胞（EC）和基质细胞（SC）置于培养中，以检测CA125的产生。培养细胞达到汇合后，异位EC中CA125的量比对数生长期时多得多。培养中的异位EC汇合后组成性分泌的CA125是在位EC的九倍。流式细胞术（FCM）分析表明，CA125表达的变化与细胞周期相关。CA125表达主要在细胞周期的G0/G1期观察到。异位EC膜中CA125表达没有扩增。SDS-PAGE和Western印迹结果表明，CA125的110 KDa分子可能是子宫腺肌病特有的。对CA125的生化和物理性质进行了检测，以表征该抗原的抗原决定簇。这些结果强烈表明，来自EC的CA125抗原决定簇由构象依赖性肽组成。我们得出结论，异位EC中CA125分泌的显著增加可能归因于子宫腺肌病患者血清CA125的升高。
