Russian Institute of Physico-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow, Russian Federation.
J Proteome Res. 2012 Jan 1;11(1):224-36. doi: 10.1021/pr2008626. Epub 2011 Dec 16.
To date, no genome of any of the species from the genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current genome sequencing technologies. Here, we report the assembly of the Spiroplasma melliferum KC3 genome into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on the discovery of 521 expressed proteins and comprehensive metabolomic profiling. A systems approach allowed us to elucidate putative pathogenicity mechanisms and to discover major virulence factors, such as Chitinase utilization enzymes and toxins never before reported for insect pathogenic spiroplasmas.
迄今为止，还没有一种螺旋体属物种的基因组被完全测序。与移动单元相似的长重复序列对当前的基因组测序技术构成了重大障碍。在这里，我们报告了蜜蜂螺旋体 KC3 基因组的组装成 4 个连续序列，随后基于发现的 521 个表达蛋白和全面的代谢组学分析进行了蛋白质基因组注释和代谢重建。系统方法使我们能够阐明潜在的致病性机制，并发现主要的毒力因子，如几丁质酶利用酶和毒素，这些从未在昆虫病原螺旋体中报道过。
