• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

自身抗原Ku与NF IV难以区分，NF IV是一种形成多聚体蛋白质-DNA复合物的蛋白质。

The autoantigen Ku is indistinguishable from NF IV, a protein forming multimeric protein-DNA complexes.

作者信息

Stuiver M H, Coenjaerts F E, van der Vliet P C

机构信息

Laboratory for Physiological Chemistry, University of Utrecht, The Netherlands.

出版信息

J Exp Med. 1990 Oct 1;172(4):1049-54. doi: 10.1084/jem.172.4.1049.

DOI:10.1084/jem.172.4.1049
PMID:2212941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2188621/
Abstract

We have isolated a cDNA encoding the 84-kD subunit of NFIV. Tryptic peptide sequences were identified within the coding sequences, confirming its proper identity. The primary sequence of the protein is identical to that of the large subunit of the Ku autoantigen. A missing NFIV peptide sequence was identified within the sequence of the small subunit of Ku. In addition, the proteins are identical in immunological aspects. We suggest that the Ku and NFIV proteins are identical. This connection adds new biochemical data to our knowledge of the Ku autoantigen.

摘要

我们分离出了一个编码NFIV 84-kD亚基的cDNA。在编码序列中鉴定出了胰蛋白酶肽序列，证实了其正确的身份。该蛋白质的一级序列与Ku自身抗原大亚基的序列相同。在Ku小亚基的序列中鉴定出了一段缺失的NFIV肽序列。此外，这两种蛋白质在免疫学方面是相同的。我们认为Ku和NFIV蛋白是相同的。这种联系为我们对Ku自身抗原的认识增添了新的生化数据。

相似文献

1
The autoantigen Ku is indistinguishable from NF IV, a protein forming multimeric protein-DNA complexes.自身抗原Ku与NF IV难以区分，NF IV是一种形成多聚体蛋白质-DNA复合物的蛋白质。
J Exp Med. 1990 Oct 1;172(4):1049-54. doi: 10.1084/jem.172.4.1049.
2
Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome.从硬皮病-多发性肌炎重叠综合征患者自身抗体识别的人类自身抗原Ku（p70/p80）80-kDa亚基蛋白编码cDNA的分离与鉴定
Proc Natl Acad Sci U S A. 1990 Mar;87(5):1777-81. doi: 10.1073/pnas.87.5.1777.
3
The nucleotide sequence of a mouse cDNA encoding the 80 kDa subunit of the Ku (p70/p80) autoantigen.编码Ku（p70/p80）自身抗原80 kDa亚基的小鼠cDNA的核苷酸序列。
Nucleic Acids Res. 1992 Jul 25;20(14):3784. doi: 10.1093/nar/20.14.3784.
4
Identification of nuclear factor IV/Ku autoantigen in a human 2D-gel protein database. Modification of the large subunit depends on cellular proliferation.
FEBS Lett. 1991 Apr 22;282(1):189-92. doi: 10.1016/0014-5793(91)80474-h.
5
Antigenic determinants of the Ku (p70/p80) autoantigen are poorly conserved between species.Ku（p70/p80）自身抗原的抗原决定簇在物种间保守性较差。
J Immunol. 1990 Dec 15;145(12):4222-8.
6
Antigenic determinants of the 70-kDa subunit of the Ku autoantigen.Ku自身抗原70-kDa亚基的抗原决定簇
Clin Immunol Immunopathol. 1992 Aug;64(2):145-52. doi: 10.1016/0090-1229(92)90192-q.
7
Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen: evidence for a family of genes encoding Ku (p70)-related polypeptides.人Ku自身抗原p70亚基的核苷酸序列及基因组结构分析：编码Ku（p70）相关多肽的基因家族的证据
Mol Biol Rep. 1992 May;16(2):91-7. doi: 10.1007/BF00419754.
8
Yolk protein factor 1 is a Drosophila homolog of Ku, the DNA-binding subunit of a DNA-dependent protein kinase from humans.卵黄蛋白因子1是人类DNA依赖性蛋白激酶的DNA结合亚基Ku在果蝇中的同源物。
J Biol Chem. 1994 Apr 15;269(15):11484-91.
9
Mapping of epitopes on the 86 kDa subunit of the Ku autoantigen.Ku自身抗原86 kDa亚基上抗原决定簇的定位
Mol Immunol. 1990 Oct;27(10):973-80. doi: 10.1016/0161-5890(90)90120-o.
10
The 86-kD subunit of Ku autoantigen mediates homotypic and heterotypic adhesion of multiple myeloma cells.Ku自身抗原的86-kD亚基介导多发性骨髓瘤细胞的同型和异型黏附。
J Clin Invest. 1998 Mar 15;101(6):1379-88.

引用本文的文献

1
The characterization and purification of a human transcription factor modulating the glutathione peroxidase gene in response to oxygen tension.一种响应氧张力调节谷胱甘肽过氧化物酶基因的人类转录因子的表征与纯化。
Mol Cell Biochem. 2002 Jan;229(1-2):73-83. doi: 10.1023/a:1017921110363.
2
Cisplatin-DNA adducts inhibit translocation of the Ku subunits of DNA-PK.顺铂 - DNA加合物抑制DNA依赖蛋白激酶（DNA-PK）的Ku亚基的易位。
Nucleic Acids Res. 2000 Dec 1;28(23):4634-41. doi: 10.1093/nar/28.23.4634.
3
DNA double-strand-break sensitivity, DNA replication, and cell cycle arrest phenotypes of Ku-deficient Saccharomyces cerevisiae.缺乏Ku蛋白的酿酒酵母的DNA双链断裂敏感性、DNA复制及细胞周期停滞表型
Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):867-72. doi: 10.1073/pnas.94.3.867.
4
Modulation of thermal induction of hsp70 expression by Ku autoantigen or its individual subunits.Ku自身抗原或其单个亚基对热诱导hsp70表达的调节作用。
Mol Cell Biol. 1996 Jul;16(7):3799-806. doi: 10.1128/MCB.16.7.3799.
5
p95vav associates with the nuclear protein Ku-70.p95vav与核蛋白Ku-70相关联。
Mol Cell Biol. 1996 Jan;16(1):37-44. doi: 10.1128/MCB.16.1.37.
6
Enhancer 1 binding factor, a Ku-related protein, is a positive regulator of RNA polymerase I transcription initiation.
Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):762-6. doi: 10.1073/pnas.91.2.762.
7
DNA-dependent protein kinase (Ku protein-p350 complex) assembles on double-stranded DNA.DNA依赖性蛋白激酶（Ku蛋白-p350复合物）在双链DNA上组装。
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6904-8. doi: 10.1073/pnas.91.15.6904.
8
A DNA-binding activity, TRAC, specific for the TRA element of the transferrin receptor gene copurifies with the Ku autoantigen.一种对转铁蛋白受体基因TRA元件具有特异性的DNA结合活性TRAC与Ku自身抗原共同纯化。
Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6354-8. doi: 10.1073/pnas.91.14.6354.
9
Production and characterization of recombinant human Ku antigen.重组人Ku抗原的制备与鉴定
Nucleic Acids Res. 1994 Sep 25;22(19):3918-24. doi: 10.1093/nar/22.19.3918.
10
A Drosophila protein homologous to the human p70 Ku autoimmune antigen interacts with the P transposable element inverted repeats.一种与人类p70 Ku自身免疫抗原同源的果蝇蛋白与P转座因子反向重复序列相互作用。
Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12681-5. doi: 10.1073/pnas.91.26.12681.

本文引用的文献

1
Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap.对多肌炎-硬皮病重叠综合征患者血清中自身抗体识别的一种高分子量酸性核蛋白的特性分析。
J Clin Invest. 1981 Sep;68(3):611-20. doi: 10.1172/jci110295.
2
Partial purification and properties of an exonuclease inhibitor induced by bacteriophage Mu-1.噬菌体Mu-1诱导产生的一种核酸外切酶抑制剂的部分纯化及性质
J Virol. 1981 Aug;39(2):548-58. doi: 10.1128/JVI.39.2.548-558.1981.
3
Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots.果蝇胚胎中一种主要核被膜蛋白的两种抗原相关形式的鉴定、发育调控及对热休克的反应：利用固定在硝酸纤维素印迹上的多肽改进抗体亲和纯化方法的应用
J Cell Biol. 1984 Jul;99(1 Pt 1):20-8. doi: 10.1083/jcb.99.1.20.
4
A 10S particle released from deoxyribonuclease-sensitive regions of HeLa cell nuclei contains the 86-kilodalton-70-kilodalton protein complex.从HeLa细胞核的脱氧核糖核酸酶敏感区域释放的10S颗粒包含86千道尔顿-70千道尔顿蛋白质复合物。
Biochemistry. 1986 Sep 9;25(18):5057-63. doi: 10.1021/bi00366a013.
5
Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders.风湿性疾病患者自身抗体识别的DNA结合蛋白抗原Ku的特性分析。
J Biol Chem. 1986 Feb 15;261(5):2274-8.
6
Mechanism of interaction between Ku protein and DNA.Ku蛋白与DNA之间的相互作用机制。
J Biol Chem. 1986 Aug 5;261(22):10375-9.
7
Isolation of a recombinant copy of the gene encoding C/EBP.编码C/EBP的基因重组拷贝的分离
Genes Dev. 1988 Jul;2(7):786-800. doi: 10.1101/gad.2.7.786.
8
HeLa nuclear protein recognizing DNA termini and translocating on DNA forming a regular DNA-multimeric protein complex.
J Mol Biol. 1989 Jul 5;208(1):65-78. doi: 10.1016/0022-2836(89)90088-0.
9
cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.Ku抗原86-kDa亚基的cDNA推导的氨基酸序列
J Biol Chem. 1989 Aug 15;264(23):13407-11.
10
Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen.编码p70（Ku）狼疮自身抗原的cDNA的分子克隆
J Biol Chem. 1989 Mar 25;264(9):5047-52.