Griffith A J, Craft J, Evans J, Mimori T, Hardin J A
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.
Mol Biol Rep. 1992 May;16(2):91-7. doi: 10.1007/BF00419754.
cDNA encoding the p70 polypeptide subunit of the human Ku autoantigen was isolated. In vitro expression analysis of the cDNA demonstrates that it encodes the entire open reading frame. Nucleotide sequence analysis and comparison to other previously described sequences indicate the existence of several single-nucleotide and amino acid polymorphisms. Southern blot analyses demonstrate the presence of multiple copies of homologous DNA sequences in the human genome. These data support the hypothesis that multiple genes encode a family of Ku(p70)-related polypeptides.
分离出编码人Ku自身抗原p70多肽亚基的cDNA。对该cDNA的体外表达分析表明，它编码完整的开放阅读框。核苷酸序列分析以及与其他先前描述序列的比较表明存在多个单核苷酸和氨基酸多态性。Southern印迹分析表明人类基因组中存在多个同源DNA序列拷贝。这些数据支持了多个基因编码一个Ku（p70）相关多肽家族的假说。
