Kuijpers Marijke J E, Heemskerk Johan W M
Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands.
Methods Mol Biol. 2012;788:3-19. doi: 10.1007/978-1-61779-307-3_1.
Intravital fluorescence microscopy is increasingly used to measure experimental arterial thrombosis in large and small arteries of mice in vivo. This chapter describes protocols for applying this technology to detect and measure thrombi formed by: (1) ultrasound-induced rupture of an atherosclerotic plaque in the carotid artery of adult Apoe (-/-) mice; (2) FeCl(3) or ligation in the carotid artery of nonatherosclerotic mice; and (3) FeCl(3) in the mesenteric venules and arterioles of young mice. In addition, we describe a protocol using two-photon laser scanning microscopy for intraluminal scanning of thrombi formed in the carotid artery. These approaches provide important information that cannot be obtained with ex vivo methods and thus are likely to lead to new insights into the complex process of thrombosis.
活体荧光显微镜越来越多地用于在体内测量小鼠大动脉和小动脉中的实验性动脉血栓形成。本章描述了应用该技术检测和测量由以下方式形成的血栓的方案：（1）超声诱导成年Apoe（-/-）小鼠颈动脉粥样硬化斑块破裂；（2）在非动脉粥样硬化小鼠的颈动脉中使用FeCl₃或结扎；（3）在幼年小鼠的肠系膜小静脉和小动脉中使用FeCl₃。此外，我们还描述了一种使用双光子激光扫描显微镜对颈动脉中形成的血栓进行腔内扫描的方案。这些方法提供了离体方法无法获得的重要信息，因此可能会为血栓形成的复杂过程带来新的见解。
