Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Anim Biotechnol. 2011 Oct;22(4):175-80. doi: 10.1080/10495398.2011.595294.
Here, we report the quantification of porcine endogenous retrovirus (PERV) copy numbers using real time PCR. After generating standard curves using plasmid DNA, copy numbers were determined for PERV pol and for a housekeeping gene, porcine estrogen receptor2 (ER2) with the same amount of genomic DNA. Using this method, we examined 6 pig breeds in Korea including two breeds of miniature pig, one domestic pig from Jeju, and imported pig breeds, Duroc, Landrace, and Yorkshire. All breeds showed PERV copy numbers ranging from 9 to 50. This method will be useful for monitoring of PERVs in a porcine xenograft.
在这里，我们使用实时 PCR 报告了猪内源性逆转录病毒 (PERV) 拷贝数的定量。使用质粒 DNA 生成标准曲线后，使用相同量的基因组 DNA 测定了 PERV pol 和管家基因猪雌激素受体 2 (ER2) 的拷贝数。使用这种方法，我们检查了韩国的 6 个猪品种，包括两个微型猪品种、济州岛的一个家猪品种和进口的猪品种杜洛克、长白猪和约克夏猪。所有品种的 PERV 拷贝数均在 9 到 50 之间。这种方法将有助于监测猪异种移植物中的 PERV。
