Cork S C, Wdks C R, Marshall R B, Fenwick S G
a Department of Veterinary Pathology and Public Health , Massey University , New Zealand.
N Z Vet J. 1994 Apr 1;42(2):74. doi: 10.1080/00480169.1994.36533.
Abstract The development of disease following oral challenge with Yersinia pseudotuberculosis (serotype 11) was compared in four groups of five birds treated with a parenteral dose of 10 mg iron dextran (Imferon), 10 mg of iron dextran plus 10 mg of the chelating agent desferrioxamine (Desferal), 10 mg of desferrioxamine or 10 mg of dextran 2 days before the experiment. Four groups of two birds received the above treatment regimens but no bacterial challenge. In iron dextran treated birds, oral challenge resulted in faecal shedding for the 10 day duration of the experiment, whereas in those birds which received dextran or desferrioxamine alone, the duration of faecal shedding was significantly less. Serological titres to the lipopolysaccharide antigen of the challenge bacteria were also lower in the groups not pretreated with iron dextran. The birds pretreated with iron dextran had diarrhoea and were clinically unwell 2 days following the initial oral challenge. Birds not given iron dextran showed no clinical signs of disease. Histological examination of five selected areas in the liver, spleen and intestine of each bird indicated that birds in the groups treated with iron dextran prior to bacterial challenge had significantly more intestinal lesions than birds in the groups not treated with iron. In contrast, there were significantly more lesions in the spleens of birds not pretreated with iron dextran. There was no evidence of stainable iron in the livers of birds challenged with Y pseudotuberculosis 10 days after an injection of 10 mg of iron dextran. This is in contrast to birds given iron dextran and no bacteria. It was concluded that pretreatment of birds with iron dextran resulted in more severe clinical disease, prolonged faecal shedding with associated intestinal lesions and higher serological titres to bacterial antigen. The number of lesions in the spleen and liver was not necessarily correlated with the severity of clinical disease, and in all infected birds the hepatic iron levels were significantly lower than in the non-infected control birds 10 days after oral challenge. It seems probable that the chicken has a high requirement for iron during infection with Y pseudotuberculosis and mobilises stored and exogenously supplied iron for tissue repair and immunological function.
比较了四组每组五只鸟在口服致病性假结核耶尔森氏菌（血清型11）后的发病情况。这四组鸟在实验前2天分别接受了以下肠外给药处理：10毫克右旋糖酐铁（Imferon）、10毫克右旋糖酐铁加10毫克螯合剂去铁胺（Desferal）、10毫克去铁胺或10毫克右旋糖酐。另有四组每组两只鸟接受上述处理方案，但未进行细菌攻毒。在接受右旋糖酐铁处理的鸟中，口服攻毒导致在实验的10天期间粪便排菌，而在仅接受右旋糖酐或去铁胺处理的鸟中，粪便排菌持续时间显著缩短。在未用右旋糖酐铁预处理的组中，针对攻毒细菌脂多糖抗原的血清学滴度也较低。用右旋糖酐铁预处理的鸟在初次口服攻毒后2天出现腹泻且临床状态不佳。未给予右旋糖酐铁的鸟未表现出疾病的临床症状。对每只鸟的肝脏、脾脏和肠道的五个选定区域进行组织学检查表明，在细菌攻毒前用右旋糖酐铁处理的组中的鸟比未用铁处理的组中的鸟有更多的肠道病变。相反，未用右旋糖酐铁预处理的鸟的脾脏中有更多病变。在注射10毫克右旋糖酐铁10天后用致病性假结核耶尔森氏菌攻毒的鸟的肝脏中没有可染色铁的证据。这与给予右旋糖酐铁但未攻毒的鸟形成对比。得出的结论是，用右旋糖酐铁对鸟进行预处理会导致更严重的临床疾病、粪便排菌时间延长并伴有相关肠道病变以及对细菌抗原的血清学滴度更高。脾脏和肝脏中的病变数量不一定与临床疾病的严重程度相关，并且在所有感染的鸟中，口服攻毒10天后肝脏铁水平显著低于未感染的对照鸟。在感染致病性假结核耶尔森氏菌期间，鸡似乎对铁有很高的需求，并调动储存的和外源供应的铁用于组织修复和免疫功能。
