Laboratory of Cancer Genetics and Production of Therapeutic Proteins, Center of Biotechnology, Sfax, Tunisia.
Acta Histochem. 2012 Oct;114(6):577-81. doi: 10.1016/j.acthis.2011.11.002. Epub 2011 Nov 30.
Cyclo-oxygenase 2 (COX-2) and E-cadherin are promising biomarkers for cancer diagnosis and therapy. The aim of this study was to examine the expression of these two proteins in primary colorectal adenocarcinomas and to investigate their association with clinicopathological characteristics including survival of patients. Immunostaining of E-cadherin and COX-2 was assessed in 70 primary colorectal adenocarcinomas from Tunisian patients. Membranous E-cadherin immunostaining and cytoplasmic COX-2 expression were observed in 74.3% and 68.6% of cases respectively. A significant association was found between COX-2 expression and age at diagnosis (P=0.02), and vessel invasion (P=0.037). The expression of E-cadherin correlated with age at diagnosis (P=0.01), and tumor size (P=0.02). In addition, by multivariate analysis, we revealed a significant association with 1-year disease free survival and a tendency with distant metastasis (P=0.017 and P=0.065 respectively). On the other hand, tumors exhibiting COX-2+/E-cadherin-profile were larger (P=0.006), and in an advanced stage (P=0.001). Survival analysis showed that COX-2 over-expression confers a reduced overall survival rate (Plog rank=0.036) and is an independent factor predictive for prognosis.
环氧化酶 2（COX-2）和 E-钙黏蛋白是癌症诊断和治疗有前途的生物标志物。本研究旨在检测这两种蛋白在原发性结直肠腺癌中的表达，并探讨其与临床病理特征（包括患者的生存）的关系。对来自突尼斯患者的 70 例原发性结直肠腺癌进行了 E-钙黏蛋白和 COX-2 的免疫染色。膜 E-钙黏蛋白免疫染色和细胞质 COX-2 表达分别在 74.3%和 68.6%的病例中观察到。COX-2 表达与诊断时的年龄（P=0.02）和血管浸润（P=0.037）之间存在显著相关性。E-钙黏蛋白的表达与诊断时的年龄（P=0.01）和肿瘤大小（P=0.02）相关。此外，通过多变量分析，我们发现与 1 年无病生存率显著相关，并与远处转移呈趋势相关（P=0.017 和 P=0.065）。另一方面，表现出 COX-2+/E-钙黏蛋白表型的肿瘤更大（P=0.006），且处于晚期（P=0.001）。生存分析显示，COX-2 过表达导致总生存率降低（对数秩检验 P=0.036），并且是预后的独立预测因素。
