Soumaoro Labile Togba, Uetake Hiroyuki, Takagi Yoko, Iida Satoru, Higuchi Tetsuro, Yasuno Masamichi, Enomoto Masayuki, Sugihara Kenichi
Department of Surgical Oncology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Dis Colon Rectum. 2006 Mar;49(3):392-8. doi: 10.1007/s10350-005-0247-x.
Several lines of experimental evidence indicated that over-expression of vascular endothelial growth factor-C and cyclooxygenase-2 genes promotes angiogenesis and lymphangiogenesis, both of which are essential for the growth and spreading of tumor cells. This study was designed to evaluate the coexpression of vascular endothelial growth factor-C and cyclooxygenase-2 in human colorectal carcinoma to determine their relationships and correlations with lymph node metastasis and prognosis.
Tissue samples of primary tumors and metastatic lymph nodes from 150 patients undergoing intentionally curative surgical resections for colorectal adenocarcinoma were immunohistochemically examined for vascular endothelial growth factor-C, cyclooxygenase-2, and CD34 expressions. Then, we analyzed their relationships and correlations with clinicopathologic findings and patients' survival time.
The positivity rate of vascular endothelial growth factor-C and cyclooxygenase-2 in the primary tumor was 68 and 72.7 percent, respectively, and in the metastatic lymph nodes was 93.3 and 80 percent, respectively. A significant correlation was found between the expression scores of vascular endothelial growth factor-C and cyclooxygenase-2 (P < 0.0001), and both also were correlated to microvessels density and several clinicopathologic parameters, including primary tumor size, lymph node metastasis, lymphatic invasion, and TNM stage. Patients with vascular endothelial growth factor-C-positive and/or cyclooxygenase-2-positive tumors had a significant shorter survival time than those with negative tumors did. However, in a multivariate analysis, only cyclooxygenase-2 expression was recognized as an independent prognostic factor (P = 0.0412; relative risk ratio, 3.067; 95 percent confidence interval, 1.046-8.994).
These data show that in human colorectal carcinoma, vascular endothelial growth factor-C and cyclooxygenase-2 are coexpressed and significantly associated with lymph node metastasis and prognosis.
多项实验证据表明，血管内皮生长因子-C（VEGF-C）和环氧化酶-2（COX-2）基因的过表达促进血管生成和淋巴管生成，这两者对于肿瘤细胞的生长和扩散至关重要。本研究旨在评估VEGF-C和COX-2在人大肠癌中的共表达情况，以确定它们与淋巴结转移及预后的关系和相关性。
对150例行根治性手术切除的结肠腺癌患者的原发肿瘤和转移淋巴结组织样本进行免疫组织化学检查，检测VEGF-C、COX-2和CD34的表达。然后，分析它们与临床病理特征及患者生存时间的关系和相关性。
原发肿瘤中VEGF-C和COX-2的阳性率分别为68%和72.7%，转移淋巴结中分别为93.3%和80%。VEGF-C和COX-2的表达评分之间存在显著相关性（P<0.0001），且二者均与微血管密度以及包括原发肿瘤大小、淋巴结转移、淋巴管浸润和TNM分期在内的多项临床病理参数相关。VEGF-C阳性和/或COX-2阳性肿瘤患者的生存时间明显短于阴性肿瘤患者。然而，在多因素分析中，只有COX-2表达被认为是独立的预后因素（P = 0.0412；相对危险比，3.067；95%置信区间，1.046 - 8.994）。
这些数据表明，在人大肠癌中，VEGF-C和COX-2共表达，且与淋巴结转移和预后显著相关。
