State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Laboratory of Advanced Materials, Fudan University, Shanghai 200433, China.
Biomaterials. 2012 Feb;33(6):1730-5. doi: 10.1016/j.biomaterials.2011.11.023. Epub 2011 Nov 30.
We herein report a material technique to control the shapes of cell nuclei by the design of the microtopography of substrates to which the cells adhere. Poly(D,L-lactide-co-glycolide) (PLGA) micropillars or micropits of a series of height or depth were fabricated, and some surprising self deformation of the nuclei of bone marrow stromal cells (BMSCs) was found in the case of micropillars with a sufficient height. Despite severe nucleus deformation, BMSCs kept the ability of proliferation and differentiation. We further demonstrated that the shapes of cell nuclei could be regulated by the appropriate micropillar patterns. Besides circular and elliptoid shapes, some unusual nucleus shapes of BMSCs have been achieved, such as square, cross, dumbbell, and asymmetric sphere-protrusion.
我们在此报告了一种通过设计细胞附着的基底的微观形貌来控制细胞核形状的材料技术。制备了一系列高度或深度的聚（D，L-乳酸-共-乙醇酸）（PLGA）微柱或微坑，发现在足够高度的微柱情况下骨髓基质细胞（BMSCs）的细胞核会发生惊人的自变形。尽管细胞核严重变形，但 BMSCs 仍然保持增殖和分化的能力。我们进一步证明，通过适当的微柱图案可以调节细胞细胞核的形状。除了圆形和椭圆形，还实现了 BMSCs 的一些不寻常的细胞核形状，如方形、十字形、哑铃形和不对称的球体突出。
