Xiangya Stomatological Hospital and Xiangya School of Stomatology, Central South University, Changsha, Hunan 410008, China.
J Healthc Eng. 2022 Jan 28;2022:7179723. doi: 10.1155/2022/7179723. eCollection 2022.
Various kinds of controlled microtopographies can promote osteogenic differentiation of mesenchymal stem cells (MSCs), such as microgrooves, micropillars, and micropits. However, the optimal shape, size, and mechanism remain unclear. In this review, we summarize the relationship between the parameters of different microtopographies and the behavior of MSCs. Then, we try to reveal the potential mechanism between them. The results showed that the microgrooves with a width of 4-60 m and ridge width <10 m, micropillars with parameters less than 10 m, and square micropits had the full potential to promote osteogenic differentiation of MSCs, while the micromorphology of the same size could induce larger focal adhesions (FAs), well-organized cytoskeleton, and superior cell areas. Therefore, such events are possibly mediated by microtopography-induced mechanotransduction pathways.
各种受控微形貌可以促进间充质干细胞（MSCs）的成骨分化，例如微沟槽、微柱和微坑。然而，最佳的形状、大小和机制尚不清楚。在这篇综述中，我们总结了不同微形貌的参数与 MSCs 行为之间的关系。然后，我们试图揭示它们之间的潜在机制。结果表明，宽度为 4-60μm、脊宽＜10μm 的微沟槽、参数小于 10μm 的微柱和方形容积微坑具有促进 MSCs 成骨分化的巨大潜力，而相同大小的微形貌则可以诱导更大的黏附斑（FA）、组织良好的细胞骨架和更大的细胞面积。因此，这些事件可能是通过微形貌诱导的机械转导途径介导的。
