Universidad Autónoma de Chihuahua, Facultad de Ciencias Químicas, Chihuahua, Posgrado en Ciencia y Tecnología de Alimentos, Chihuahua, México.
J Food Sci. 2012 Jan;77(1):C89-94. doi: 10.1111/j.1750-3841.2011.02468.x. Epub 2011 Dec 2.
Solanum elaeagnifolium (trompillo or silverleaf nightshade) is an endemic plant from the northeast of Mexico and southwest of United States. This plant is considered as a weed with negative impact on agriculture and livestock production. Nevertheless, in some places of Chihuahua, Mexico, the berries of this plant have been used for decades in the manufacture of artisanal filata-type asadero cheese. The milk-clotting enzyme of S. elaeagnifolium has been scarcely studied; for this reason, the aim of this work was to explore some properties of this plant coagulant. Protein extracts (PEs) from ripe berries of S. elaeagnifolium were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and assessed for milk-clotting activity. In addition, milk gels and miniature fresh-type cheeses manufactured with the plant coagulant were analyzed for their texture properties. The PE from the berries of S. elaeagnifolium contained 8 proteins with molecular weights from 22 to 62 kDa. Some bands observed in the PE had similar molecular weights as reported for aspartic proteinases such as chymosin. The extracts from the berries of S. elaeagnifolium had lower milk-cloting activities than observed with rennin or chymosin, but this plant coagulant produced firm gels under acidic conditions. The mini-cheeses manufactured with this coagulant were softer than cheeses manufactured with rennin or chymosin. For this reason, the coagulant from the ripe berries of S. elaeagnifolium could be suitable not only for the manufacture of filata-type cheeses but also for the manufacture of soft cheeses such as cream cheese.
Silverleaf nightshade (trompillo) is a plant that grows in northern Mexico and the southwestern United States. This plant is considered a weed with negative impact on agriculture and livestock production. However, the ripe berries of this plant have been used by ancient Pima Indians as a substitute of rennin in making cheese. In this work, it was observed that this plant coagulant had lower activity and produced softer cheeses than did rennin or chymosin. For this reason, the coagulant from berries of S. elaeagnifolium could be used for the manufacture of soft cheeses such as cream cheese.
Solanum elaeagnifolium（ Trompillo 或银叶龙葵）是一种来自墨西哥东北部和美国西南部的特有植物。这种植物被认为是一种对农业和畜牧业生产有负面影响的杂草。然而，在墨西哥奇瓦瓦州的一些地方，这种植物的浆果已经被用于制造传统的丝状 asadero 奶酪数十年。S. elaeagnifolium 的凝乳酶很少被研究；因此，这项工作的目的是探索这种植物凝结剂的一些特性。用成熟浆果的蛋白质提取物（PE）进行 SDS-PAGE 分析，并评估其凝乳活性。此外，还分析了用植物凝结剂制成的牛奶凝胶和微型新鲜型奶酪的质地特性。S. elaeagnifolium 浆果的 PE 中含有 8 种分子量为 22 至 62 kDa 的蛋白质。在 PE 中观察到的一些条带与凝乳酶或 chymosin 等天冬氨酸蛋白酶具有相似的分子量。与凝乳酶或 chymosin 相比，S. elaeagnifolium 浆果提取物的凝乳活性较低，但这种植物凝结剂在酸性条件下可产生坚固的凝胶。用这种凝结剂制成的微型奶酪比用凝乳酶或 chymosin 制成的奶酪柔软。因此，成熟浆果的凝结剂不仅适合制造丝状奶酪，也适合制造奶油奶酪等软奶酪。
银叶龙葵（ Trompillo）是一种生长在墨西哥北部和美国西南部的植物。这种植物被认为是一种对农业和畜牧业生产有负面影响的杂草。然而，这种植物的成熟浆果被古代皮马印第安人用作制造奶酪的凝乳酶替代品。在这项工作中，观察到这种植物凝结剂的活性较低，制成的奶酪比凝乳酶或 chymosin 制成的奶酪柔软。因此，S. elaeagnifolium 浆果的凝结剂可用于制造奶油奶酪等软奶酪。
