Gutiérrez-Méndez Néstor, Balderrama-Carmona Alejandro, García-Sandoval Socorro E, Ramírez-Vigil Pamela, Leal-Ramos Martha Y, García-Triana Antonio
Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Chihuahua 31125, Mexico.
Foods. 2019 Jan 30;8(2):44. doi: 10.3390/foods8020044.
Cream cheese is a fresh acid-curd cheese with pH values of 4.5⁻4.8. Some manufacturers add a small volume of rennet at the beginning of milk fermentation to improve the texture of the cream cheese. However, there is no information about the effect that proteases other than chymosin-like plant-derived proteases may have on cream cheese manufacture. This work aimed to describe some proteolytic features of the protease extracted from fruits of Cavanilles and to assess the impact that this plant coagulant has on the viscoelastic properties of cream cheeses. Results showed that caseins were not hydrolyzed extensively by this plant-derived coagulant. In consequence, the ratio of milk clotting units () to proteolytic activity () was higher (1184.4 /) than reported for other plant proteases. The plant coagulant modified neither yield nor composition of cream cheeses, but viscoelastic properties did. Cream cheeses made with chymosin had a loss tangent value ( δ = 0.257) higher than observed in cheeses made with 0.8 mL of plant-derived coagulant per liter ( δ = 0.239). It is likely that casein fragments released by the plant-derived coagulant improve the interaction of protein during the formation of acid curds, leading to an increase in the viscoelastic properties of cream cheese.
奶油奶酪是一种新鲜的酸凝乳奶酪,pH值为4.5⁻4.8。一些制造商在牛奶发酵开始时添加少量凝乳酶,以改善奶油奶酪的质地。然而,关于除凝乳酶样植物源蛋白酶之外的其他蛋白酶对奶油奶酪制造可能产生的影响,尚无相关信息。这项工作旨在描述从卡瓦尼利亚斯果实中提取的蛋白酶的一些蛋白水解特性,并评估这种植物凝固剂对奶油奶酪粘弹性的影响。结果表明,这种植物源凝固剂不会广泛水解酪蛋白。因此,凝乳单位()与蛋白水解活性()的比率(1184.4 /)高于其他植物蛋白酶的报道值。这种植物凝固剂既没有改变奶油奶酪的产率,也没有改变其成分,但却改变了其粘弹性。用凝乳酶制作的奶油奶酪的损耗角正切值(δ = 0.257)高于每升使用0.8 mL植物源凝固剂制作的奶酪(δ = 0.239)。植物源凝固剂释放的酪蛋白片段可能会改善酸性凝乳形成过程中蛋白质之间的相互作用,从而导致奶油奶酪的粘弹性增加。