National Institute of Livestock and Grassland Science, Nasushiobara-shi, Tochigi-ken 329-2793, Japan.
Meat Sci. 2012 Mar;90(3):807-13. doi: 10.1016/j.meatsci.2011.11.019. Epub 2011 Nov 12.
In this experiment, we studied the effects of the dietary roughage/concentrate ratio on the expression of the angiogenic growth factor (VEGF and FGF-2) and the adipogenic transcription factor (C/EBPβ, C/EBPα, and PPARγ) gene in the adipose tissues of Wagyu steers. Steers were fed a high-roughage diet (R group, 35% roughage and 65% concentrate on a TDN basis) or a high-concentrate diet (C group, 10% roughage and 90% concentrate) during the entire fattening period (from 10 to 30months of age) with the same amount of TDN intake between groups. In mesenteric and intermuscular adipocytes, the expression of the angiogenic growth factors was higher in the R group than in the C group. In contrast, the expression of adipogenic transcription factors in the subcutaneous and intramuscular adipocytes was higher in the C group than in the R group. These results indicate that the dietary roughage/concentrate ratio affects the fat depot-specific differences in the angiogenic and adipogenic gene expression pattern.
在本实验中，我们研究了饲粮粗饲料/精饲料比例对瘤胃旁路手术后的大鼠血管生成生长因子（VEGF 和 FGF-2）和脂肪生成转录因子（C/EBPβ、C/EBPα 和 PPARγ）基因在脂肪组织中表达的影响。在整个育肥期（从 10 到 30 月龄），牛只均采食相同的总能（TDN）摄入量，一组采食高粗饲料饲粮（R 组，粗饲料 35%，精饲料 65%），另一组采食高精饲料饲粮（C 组，粗饲料 10%，精饲料 90%）。在肠系膜和肌间脂肪细胞中，R 组的血管生成生长因子表达高于 C 组。相比之下，C 组的皮下和肌内脂肪细胞中脂肪生成转录因子的表达高于 R 组。这些结果表明，饲粮粗饲料/精饲料比例影响血管生成和脂肪生成基因表达模式的脂肪沉积特异性差异。
